LOCUS       Exported                3085 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Regulator-reporter vector pWUR873, complete sequence.
ACCESSION   KX618638
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   van Rossum T, Muras A, Baur MJ, Creutzburg SC, van der Oost J, 
            Kengen SW.
  TITLE     A growth- and bioluminescence-based bioreporter for the in vivo 
            detection of novel biocatalysts
  JOURNAL   Microb Biotechnol (2017) In press
  PUBMED    28393499
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Creutzburg SCA., Kengen SWM., van der Oost J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUL-2016) Laboratory of Microbiology, Wageningen 
            University and Research, Stippeneng 4, Wageningen 6708 WE, The 
            Netherlands
REFERENCE   3  (bases 1 to 3085)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Regulator-reporter vector pWUR873"
                     /mol_type="other DNA"
                     /db_xref="taxon:1979217"
     promoter        105..123
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      105..121
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     regulatory      171..176
                     /regulatory_class="ribosome_binding_site"
                     /note="RBS"
     CDS             185..901
                     /codon_start=1
                     /gene="gfpuv"
                     /product="GFPuv"
                     /label=gfpuv
                     /protein_id="ARH53380.1"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     gene            185..901
                     /gene="gfpuv"
                     /label=gfpuv
     regulatory      908..1036
                     /regulatory_class="terminator"
                     /note="T7 terminator"
     terminator      971..1018
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1222..2082)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="Bla"
                     /protein_id="ARH53381.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(1222..2082)
                     /gene="bla"
                     /label=bla
     promoter        complement(2083..2174)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2509..3054)
                     /direction=LEFT
                     /label=p15A
                     /note="p15A"