LOCUS       Exported               13103 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pWSMV-S1RN, complete sequence.
ACCESSION   AF329446
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13103)
  AUTHORS   Stenger DC, French R, Choi I-R.
  TITLE     pWSMV-S1RN: an improved plant virus vector to express foreign genes 
            in cereals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13103)
  AUTHORS   Stenger DC, French R, Choi I-R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2000) USDA-ARS, University of Nebraska-Lincoln, 
            344 Keim Hall, Lincoln, NE 68583, USA
REFERENCE   3  (bases 1 to 13103)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13103
                     /organism="Expression vector pWSMV-S1RN"
                     /focus
                     /mol_type="genomic DNA"
                     /note="modified from infectious clone (pWSMV-ACYC) of wheat
                     streak mosaic virus; engineered to accept inserts at unique
                     SalI site; cloned into plasmid vector pACYC-177 modified to
                     include an upstream SP6 promoter and downstream NotI site 
                     to linearize transcription template"
                     /db_xref="taxon:153506"
     source          1..9492
                     /organism="Wheat streak mosaic virus"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:31741"
     CDS             131..9241
                     /codon_start=1
                     /product="polyprotein"
                     /label=polyprotein
                     /protein_id="AAK16492.1"
                     /translation="MATANCLLGDFGRQGVVANDPYVQCRARTLIFLSTEEEVDVVVNH
                     HGPGSIFWSKEGILTQTAKNLYKATAYGLGYDLAANVFVCGKCRSSCTQYRYFIEDHFA
                     CDKLVEKNCAYIKDDKYVKVVEAFPIMPSYATPGQETRIIQWMNKTSQCLADHCIQRTR
                     EITFTNSKTQEEETRVKDCSLEVFYDDFDEAHAVIEHAHRKNPVHEYKEKQLRMTSNNI
                     AALVDQVTRMMHSKGKTVEIVGSKGHKKFAKIPLKHTMGYPKRDWDATKDIPEDFRGFI
                     TTYSGVIQYTRKVQDHEVTLGWSGVLLSEMDVPDGYQEDCVDGLFIVMGRCAHGRIQNA
                     LKPKCTHGLRWYGVDQAVNKVIPKYHDVCNNNCASYLEALPRKVSRVWQSMFDIHNLRC
                     DQCRTEWKMRTASEHLQLLQKSVEHYMQTYPDSDVTLFKAFLQALGPDEEVEARQLKPN
                     NTLQLVDVWRTMKNTINIPNRIIYMGMFTDNYGNFDFFPNTSMPELFAMHMQPVQHKML
                     EDGTIETNFRFVDLEGKIQTSIESLYPTFDSTYWNEQARRVHRIQPLADCSMEINGESK
                     PICHWEGNVPLYNPIIRATPGQLPFGVTTHLLSVNDRSGRFHYVPKNGYCYMYIFACAM
                     IFCGNSNRSTVDAFVRQVCEDLGPWPMFGDVLRQLDWMATFYGCYDALVPVILVDHTRK
                     TMHVPTPYGIKQSGMHTIRVNTVLELITLDTMASGAMKDYKIGGFQETVLSIQACVKSR
                     KEFVRKINKDAEWLVDMFINPSTLFVLGGLIEVHQVILADVENSFDKSAALLNLRQIAL
                     KLGPHLESKQRVRQYMELMIQHRASVEAIIPSQHMKAEMMQYIDALQRSILEEQVIIEM
                     DRVGGKEKMLVEQDLSHAECAYNEFFNSIGYLNFHGTVLRLTYSGPGRKVGEVLESLRD
                     NWLTRYLRGPKQPRDYKGSSLRIWRKVTHLCGNAYRWVFYNMAANVLQVILIGLSTVFG
                     AYLLKKILKMLQWEKEQESTELVEYQGKREEAWITRVMAVLYIIASLFSVDFSSALYSN
                     LVKFRTIFDILKFNCEYQSGIFESLKNQLGNIPAFHEVHLYDHEATQVAVPPAILTFER
                     WFETRITSGQQGYAPLDGSHVSLTMTKDTVGEIATQVQTHKAKEFLIIGHVGCGKSTAF
                     PATLSRNGRVMICEPTRVLVTNLQDSMLATRNLSISAMMRNHRVMTASNITVTTYGYAL
                     HYLYNNSHNLSEYDYILFDEVHQTSAEMLVFYNWLKSTTWEGKLIKLTATNNTVNGDMQ
                     TQQALDVKTWPVMDHRTFMQEQGRGTAHDASTLGDVIIVFLTSFREIDESADILSKNSK
                     IGVIKADSRHLRNKISLMDDVEALRAEKKYILATNILQNGVNLHADVVVDFGFKIVPAI
                     DSDNRMITVKRQLINKSDRIQRLGRVGRMKMGYARKIGNEIDASFALDEVTATEAALLA
                     FGLGVAPVLQNVDQHTFGKITAEQVRTAARFEMQLSYMVWMINRDGTMATRLYEQFKSL
                     LLTPGNTSLAPYYETLVDTHRFRTIGQYATLGYMRTDEKHHLVLPFHHNDVSVEFAERI
                     GEAYMASQVPTSIKLRVPAVNHREVAMKMSANPEDVGTILYMVEQALISEKTKLENLTQ
                     AFQQQQSTYCSVLIPNFNVAGRLTQAMDRIRKNVSVLQHQKTALEKAAVTYDYTKLVEL
                     LDENPSIASHVSYQAGPAKFIDEFILEKRDYGWLPYLAVGTACAIAGTTLVMMYYRRMK
                     RSVKFEGKAARNRSAKRQSARDQKMERGNEYTYYDAGDTLYNGVQENMNHAPDWTDRIK
                     KKTHAYAMQFGREVPKTETQRSSQYWHFYGFDPKMYDSVEFKDIAANFSVHQDAKAMDL
                     QKAFTEMVENRWDDEDFFDEKIPKRVLAIFRKGDKVREVALAPHKPNQVNKRGLPVGHA
                     DHRGEWRQTQPSFEKEVSYENKSTFEGARSLDHIHQNQVILVEDNQQLNGLIVGNILLA
                     PYHFTRGMRNREEKETRMLTQFGTYNLGKLTNKHVTKFTMMDLVALTLPPTFQARRKLK
                     CFRPPREGERAMLVTMQYEKAGWVAKQSAETTITPFGDRHDGLWKHRISTGPGDCGSAI
                     VAVADLKVVGFHNLGGKGENYFTPITIEVMDFLAEKSVTPLVPWKFSDEQVDLCGLIAA
                     NGADKYPFTKTISDLVSWQSLQMTKYCGENFKAIAYAPNRMSKRHVITGKRPEFIKFLD
                     SHPKWNATVTPFLNGFQPSVLTHEAYYKDVLKYNKDIIVGGTDEVCFAKAVVATIGILE
                     IAGFSKGQFQPIFDGCKIFNDLNLDAAMGALYSGKKSAYFDGATSDEINEFFELSAAKL
                     LSNGHGVWSGLLKAELRPKAKVVANKTRTFTSAPIDILMGAKAVVDEFNKFFYTKHLRG
                     PWTVGINKFNGGWDLLAKNLMVHEWFIDADGSQFDSSITPLLMNAILNIRQYFMAEDDE
                     AEQMLANLYTQIINTCILIEDGTIVQKFRGNNSGQPSTVVDNTMCLIIAMEYCRMRVEK
                     DHEHRMRILYVCNGDDLLINADTKDKDFIQQYFADYMRELELNYSFDEAYRSIEEVEYM
                     SHTFMKRNSMYIPKLKRERIVAILEWQRSKEPKAIQSAIIAAYVEAFGYDEFTEMIEEL
                     AQEVSAVWPDFKLPSRQEVEDLYLTGTRTDLGEEIKECGEQYCVYESSEAATDAVLAAA
                     NAGTGSASSSGSTQSSQSASTASGSGSSPSGSGSGAAGGSGSGSAQTQSNNVSVMAGLD
                     TGGAKTDQGSGSKGTGGSFTSNPVRTGGRATDVQDQTPGLVFPAPKITTKAIYMPKTVR
                     DKIKPEMINNMIKYQPRAELIDNRYATTEQLNTWIKEASEGLDVTEDVFINTLLPGWVY
                     HCIINTTSPENRALGTWRVVNNAGKDNEQQLEFKIEPMYKAAKPSLRAIMRHFGEGARV
                     MIEESVRIGKPIIPRGFDKAGVLSINNIVAACDFIMRGADDTPNFVQVQNSVAVNRLRG
                     IQNKLFAQARLSAGTNEDNSRHDADDVRENTHSFNGVNALA"
     misc_feature    1190..1195
                     /label=unique SalI site
                     /note="unique SalI site"
     rep_origin      complement(9766..10311)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(10476..11336)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11337..11441)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        13086..1
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"