LOCUS       Exported                6928 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pWS32, complete sequence.
ACCESSION   AF433043
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6928)
  AUTHORS   Sundaresan V, Springer P, Volpe T, Haward S, Jones JD, Dean C, Ma H,
            Martienssen R.
  TITLE     Patterns of gene action in plant development revealed by enhancer 
            trap and gene trap transposable elements
  JOURNAL   Genes Dev. 9 (14), 1797-1810 (1995)
  PUBMED    7622040
REFERENCE   2  (bases 1 to 6928)
  AUTHORS   de la Bastide M, Preston R, OShaughnessy A, May B, Sundaresan V, 
            Martienssen R, McCombie WR.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2001) Cold Spring Harbor Laboratory, 1 Bungtown 
            Rd, Cold Spring Harbor, NY 11724, USA
REFERENCE   3  (bases 1 to 6928)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6928
                     /organism="Cloning vector pWS32"
                     /mol_type="other DNA"
                     /db_xref="taxon:176660"
     mobile_element  64..1852
                     /mobile_element_type="transposon:Ds transposable element"
                     /note="5 fragment"
     promoter        complement(1891..2271)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             2376..3170
                     /codon_start=1
                     /gene="npt2"
                     /product="neomycin phosphotransferase"
                     /label=npt2
                     /protein_id="AAL30402.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            2376..3170
                     /gene="npt2"
                     /label=npt2
     terminator      3344..4051
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     terminator      4231..4483
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4550..6361)
                     /codon_start=1
                     /gene="uidA"
                     /product="beta-glucuronidase"
                     /label=uidA
                     /protein_id="AAL30403.1"
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(4550..6361)
                     /gene="uidA"
                     /label=uidA
     intron          complement(6364..6615)
                     /note="synthetic GPA1 intron containing splice donors and 
                     acceptors in 3 reading frames"
     mobile_element  6511..6702
                     /mobile_element_type="transposon:Ds transposable element"
                     /note="3 fragment; transferred to T-DNA vector pAJ8 for 
                     transformation of Arabidopsis thaliana"
     mobile_element  6720..6911
                     /mobile_element_type="transposon:Ds transposable element"
                     /note="3 fragment"