LOCUS       Exported                6372 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pWH1274, complete sequence.
ACCESSION   JN381160
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Wang Y, Chen Y, Zhou Q, Huang S, Ning K, Xu J, Kalin RM, Rolfe S, 
            Huang WE.
  TITLE     A culture-independent approach to unravel uncultured bacteria and 
            functional genes in a complex microbial community
  JOURNAL   PLoS ONE 7 (10), E47530 (2012)
  PUBMED    23082176
REFERENCE   2  (bases 1 to 6372)
  AUTHORS   Wang Y, Huang W.
  TITLE     Development of a toolbox for the dissection of naphthalene 
            degradation genes from unculturable microorganisms in complex 
            environment
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6372)
  AUTHORS   Wang Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUL-2011) Kroto Research Institute, University of 
            Sheffield, Broad Lane, Sheffield, South Yorkshire S3 7HQ, United 
            Kingdom
REFERENCE   4  (bases 1 to 6372)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Cloning vector pWH1274"
                     /mol_type="other DNA"
                     /note="shuttle plasmid for Acinetobacter and Escherichia 
                     coli"
                     /db_xref="taxon:1118296"
     regulatory      10..38
                     /regulatory_class="promoter"
                     /note="Ptet promoter"
     promoter        10..38
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             86..1276
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance protein"
                     /label=tetR
                     /protein_id="AEU12410.1"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     gene            86..1276
                     /gene="tetR"
                     /label=tetR
     misc_feature    4219..4359
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4530..5144)
                     /direction=LEFT
                     /label=Ori for Escherichia coli
                     /note="Ori for Escherichia coli"
     rep_origin      complement(4545..5133)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5304..6164)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /function="ampicillin resistance"
                     /label=bla
                     /protein_id="AEU12411.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(5304..6164)
                     /gene="bla"
                     /label=bla
     promoter        complement(6165..6269)
                     /gene="bla"
                     /label=AmpR promoter