LOCUS       Exported                8179 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pWEB, complete sequence.
ACCESSION   AF075573
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8179)
  AUTHORS   Fiandt M, Jendrisak J, Meis R.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-1998) R&D, Epicentre Technologies, 1202 Ann St., 
            Madison, WI 53713, USA
REFERENCE   2  (bases 1 to 8179)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8179
                     /organism="Cloning vector pWEB"
                     /mol_type="genomic DNA"
                     /note="derived from cosmid cloning vector pWE-15 deposited 
                     under GenBank Accession Number X65279; Sma I site of pWE-15
                     has been removed and a new multiple cloning region added"
                     /db_xref="taxon:78646"
     primer_bind     12..35
                     /label=M13 forward primer binding site
                     /note="M13 forward primer binding site"
     primer_bind     complement(50..72)
                     /label=T7 promoter primer binding site
                     /note="T7 promoter primer binding site"
     promoter        complement(54..72)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        94..122
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     misc_feature    460..935
                     /label=lambda phage cos site
                     /note="lambda phage cos site"
     promoter        3287..3616
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3467..3602
                     /label=simian virus 40 origin of replication
                     /note="simian virus 40 origin of replication"
     CDS             3973..4767
                     /codon_start=1
                     /gene="neo"
                     /product="aminoglycoside 3-phosphotransferase"
                     /function="neomycin/kanamycin resistance"
                     /label=neo
                     /note="neomycin-kanamycin phosphotransferase type II"
                     /protein_id="AAC26829.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            3973..4767
                     /gene="neo"
                     /label=neo
     intron          5185..5250
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5380..5400
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5825..5959
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(6162..7052)
                     /direction=LEFT
                     /label=Col E1 origin of replication
                     /note="Col E1 origin of replication"
     rep_origin      complement(6355..6943)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7114..7974)
                     /codon_start=1
                     /gene="amp"
                     /product="beta-lactamase"
                     /function="ampicillin resistance"
                     /label=amp
                     /protein_id="AAC26830.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(7114..7974)
                     /gene="amp"
                     /label=amp
     promoter        complement(7975..8079)
                     /gene="bla"
                     /label=AmpR promoter