LOCUS       Exported                7478 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pWDYcos1.0.1, complete sequence.
ACCESSION   AF129504
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7478)
  AUTHORS   Yeh RK, Dunn DM, Weiss RB.
  TITLE     Low-copy number, plasmid R1 cosmid vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7478)
  AUTHORS   Yeh RK, Dunn DM, Weiss RB.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-1999) Human Genetics, University of Utah, 2030 
            East 20 South, Salt Lake City, UT 84112, USA
REFERENCE   3  (bases 1 to 7478)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7478
                     /organism="Cloning vector pWDYcos1.0.1"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:92869"
     CDS             complement(74..787)
                     /codon_start=1
                     /product="lambda cI857 repressor"
                     /label=lambda cI857 repressor
                     /protein_id="AAD33415.1"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     regulatory      835..870
                     /regulatory_class="promoter"
                     /function="over-expression of RepA at 42C"
                     /note="lambda pR"
     RBS             876..884
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3 end of the 16S rRNA (Chen et al., 1994)"
     CDS             1040..1300
                     /codon_start=1
                     /gene="copB"
                     /product="CopB"
                     /label=copB
                     /protein_id="AAD33416.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEIK
                     VFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1040..1300
                     /gene="copB"
                     /label=copB
     misc_RNA        1416..1507
                     /label=copA antisense RNA
                     /note="copA antisense RNA"
     CDS             1592..2449
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /protein_id="AAD33414.1"
                     /translation="MTDLHQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSRF
                     DFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECGLA
                     TESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTLALFAALDVSED
                     AVAAARRSRVEWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIKRARA
                     RRDANRERQDIVTLVKRQLTREISEGRFTANGEAVKREVERRVKERMILSRNRNYSRLA
                     TASP"
     gene            1592..2449
                     /gene="repA"
                     /label=repA
     rep_origin      2625..3416
                     /label=oriR
                     /note="oriR"
     protein_bind    3491..3512
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3527..3557
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3565..3581
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3589..3605
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3778..3882
                     /note="multiple cloning and insert-release sites; contains 
                     NotI-BamHI-NotII restriction sites"
     misc_feature    3884..6374
                     /label=double cos sites
                     /note="double cos sites"
     promoter        6403..6507
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6508..7368
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactmase"
                     /label=bla
                     /protein_id="AAD33413.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDPGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            6508..7368
                     /gene="bla"
                     /label=bla