LOCUS       Exported                6137 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pWB104, complete sequence.
ACCESSION   EF216321
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6137)
  AUTHORS   Bourn WR, Shephard E, Powels R, Stutz H, Douglas N, Jaffray A, 
            Williamson A-L.
  TITLE     The use of guided evolution to create a stable, immunogenic HIV-1 
            antigen expressing recombinant BCG
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6137)
  AUTHORS   Bourn WR.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2007) Medical Virology and Institute of Infectious
            Disease and Molecular Medicine, University of Cape Town, Anzio Road 
            Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 6137)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6137
                     /organism="Expression vector pWB104"
                     /mol_type="other DNA"
                     /db_xref="taxon:423229"
     CDS             120..935
                     /codon_start=1
                     /gene="aph"
                     /product="glycoside-3-phosphotransferase"
                     /label=aph
                     /note="kanamycin resistance"
                     /protein_id="ABN14094.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            120..935
                     /gene="aph"
                     /label=aph
     rep_origin      1222..2009
                     /note="derived from Escherichia coli; OriE"
     rep_origin      1267..1855
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      2014..3909
                     /note="mycobacterial origin; OriM"
     regulatory      3936..4022
                     /regulatory_class="promoter"
                     /note="BCG hsp60"
     regulatory      4046..4052
                     /regulatory_class="ribosome_binding_site"
                     /note="weak synthetic Shine Delgarno sequence; RBSC"
     gene            4088..4450
                     /gene="tat"
                     /label=tat
                     /note="shuffled BCG codon optimized tat gene from HIV-1 
                     subtype C isolate"
     gene            4460..5929
                     /gene="gag"
                     /label=gag
                     /note="BCG codon optimized gag gene from HIV-1 subtype C 
                     isolate"
     terminator      6055..6101
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     regulatory      6058..6101
                     /regulatory_class="terminator"
                     /note="rrnBT1"