LOCUS       Exported                9231 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pVZ326, complete sequence.
ACCESSION   MG356711
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9231)
  AUTHORS   Obando ST, Babykin MM, Zinchenko VV.
  TITLE     A Cluster of Five Genes Essential for the Utilization of 
            Dihydroxamate Xenosiderophores in Synechocystis sp. PCC 6803
  JOURNAL   Curr. Microbiol. (2018) In press
  PUBMED    29785634
REFERENCE   2  (bases 1 to 9231)
  AUTHORS   Obando TSA., Babykin MM, Zinchenko VV.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2017) Dept. of Genetics, Moscow State University, 
            Moscow 119991, Russian Federation
REFERENCE   3  (bases 1 to 9231)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9231
                     /organism="Cloning vector pVZ326"
                     /mol_type="other DNA"
                     /note="derived from cloning vector pVZ321, GenBank 
                     Accession Number AF100176"
                     /db_xref="taxon:2259398"
     misc_feature    1..1699
                     /note="region derived from transposon Tn903, GenBank 
                     Accession Number J01839"
     CDS             complement(422..1237)
                     /codon_start=1
                     /gene="aphA"
                     /product="aminoglycoside 3-phosphotransferase"
                     /function="kanamycin resistanse"
                     /label=aphA
                     /protein_id="AWZ62435.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(422..1237)
                     /gene="aphA"
                     /label=aphA
     misc_difference 1239..1240
                     /replace="ac"
                     /compare="AF100176.1"
                     /note="creates NdeI site overlapping the ATG start codon of
                     aphA gene"
     misc_feature    1700..7521
                     /note="region derived from the plasmid RSF1010, GenBank 
                     Accession Number M28829"
     rep_origin      2095..2489
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     CDS             complement(2516..2800)
                     /codon_start=1
                     /gene="mobC"
                     /product="mobilization protein C"
                     /label=mobC
                     /protein_id="AWZ62436.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
     gene            complement(2516..2800)
                     /gene="mobC"
                     /label=mobC
     oriT            2831..2918
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             2999..5128
                     /codon_start=1
                     /gene="mobA"
                     /product="mobilization protein A"
                     /label=mobA
                     /protein_id="AWZ62437.1"
                     /translation="MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAESG
                     HMPEFVERPADYWDAADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGAER
                     LPYTLAIHAGGGENPHCHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEALKPK
                     AWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGRGIRT
                     DRADVALNIDTANAQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEHGDTGR
                     RSPAGHEPDPAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERPERVAGV
                     ALEAMANRDAGFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRTLQAIGRQ
                     LKAMGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHG
                     LVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEY
                     DADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQI
                     EQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQK
                     LASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPA
                     PRQRGMDRGGPDFSM"
     gene            2999..5128
                     /gene="mobA"
                     /label=mobA
     CDS             3747..4160
                     /codon_start=1
                     /gene="mobB"
                     /product="mobilization protein B"
                     /label=mobB
                     /protein_id="AWZ62438.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
     gene            3747..4160
                     /gene="mobB"
                     /label=mobB
     CDS             4157..5128
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B"
                     /label=repB
                     /protein_id="AWZ62439.1"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     gene            4157..5128
                     /gene="repB"
                     /label=repB
     CDS             5189..5401
                     /codon_start=1
                     /product="protein E"
                     /label=protein E
                     /protein_id="AWZ62440.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
     CDS             5403..5609
                     /codon_start=1
                     /gene="cac"
                     /product="repressor protein F"
                     /label=cac
                     /protein_id="AWZ62441.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
     gene            5403..5609
                     /gene="cac"
                     /label=cac
     CDS             5639..6478
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A"
                     /label=repA
                     /protein_id="AWZ62442.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     gene            5639..6478
                     /gene="repA"
                     /label=repA
     CDS             6465..7316
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C"
                     /label=repC
                     /protein_id="AWZ62443.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     gene            6465..7316
                     /gene="repC"
                     /label=repC
     misc_feature    7522..8687
                     /note="region derived from the plasmid pBR325, GenBank 
                     Accession Number L08855"
     promoter        complement(8064..8168)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8371..8473
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             8474..9133
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /function="chloramphenicol resistance"
                     /label=cat
                     /protein_id="AWZ62444.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            8474..9133
                     /gene="cat"
                     /label=cat
     misc_feature    8688..9219
                     /note="region derived from transposon Tn9, GenBank 
                     Accession Number J01841, V00622"