LOCUS       Exported                9152 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Chloroplast transformation vector pVSR326, complete sequence.
ACCESSION   AF527485
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9152)
  AUTHORS   Reddy VS, Giovanni F, Leelavathi S.
  TITLE     Chloroplast transformation vector pVSR326
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9152)
  AUTHORS   Reddy VS, Giovanni F, Leelavathi S.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2002) Plant Transformation Group, International 
            Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali 
            Marg, New Delhi, Delhi 110067, India
REFERENCE   3  (bases 1 to 9152)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9152
                     /organism="Chloroplast transformation vector pVSR326"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:202795"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    405..1636
                     /note="chloroplast targeting sequence 1; derived from 
                     tobacco"
     regulatory      1661..2132
                     /regulatory_class="promoter"
                     /note="psbA promoter; derived from rice"
     CDS             2133..3944
                     /codon_start=1
                     /gene="uidA"
                     /product="beta-glucuronidase"
                     /label=uidA
                     /note="reporter gene"
                     /protein_id="AAM92009.1"
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFSLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            2133..3944
                     /gene="uidA"
                     /label=uidA
     regulatory      4014..4387
                     /regulatory_class="terminator"
                     /note="psbA terminator; derived from rice"
     regulatory      4393..4503
                     /regulatory_class="promoter"
                     /note="rrn promoter; derived from rice"
     CDS             4527..5321
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycosideadeninetransferase"
                     /label=aadA
                     /note="selection marker"
                     /protein_id="AAM92010.1"
                     /translation="MAREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPA
                     KRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDL
                     FEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQ
                     PVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            4527..5321
                     /gene="aadA"
                     /label=aadA
     regulatory      5326..5583
                     /regulatory_class="terminator"
                     /note="rbcL terminator; derived from rice"
     misc_feature    5598..6914
                     /note="chloroplast targeting sequence 2; derived from 
                     tobacco"
     primer_bind     complement(6931..6947)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6955..6971
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6979..7009)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7024..7045
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7333..7921)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8092..8952)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8953..9057)
                     /gene="bla"
                     /label=AmpR promoter