LOCUS       Exported               11368 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pVMGCRT85, complete sequence.
ACCESSION   EU232662
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11368)
  AUTHORS   Gao M, Teplitski M.
  TITLE     RIVET-a tool for in vivo analysis of symbiotically relevant gene 
            expression in Sinorhizobium meliloti
  JOURNAL   Mol. Plant Microbe Interact. 21 (2), 162-170 (2008)
  PUBMED    18184060
REFERENCE   2  (bases 1 to 11368)
  AUTHORS   Gao M, Teplitski M.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2007) Soil and Water Science, University of 
            Florida-IFAS, 1376 Mowry Rd., Cancer & Genetics Research Center, 
            Gainesville, FL 32611, USA
REFERENCE   3  (bases 1 to 11368)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11368
                     /organism="Cloning vector pVMGCRT85"
                     /mol_type="other DNA"
                     /label=portable RIVET plasmid
                     /note="portable RIVET plasmid"
                     /db_xref="taxon:481818"
     regulatory      28..58
                     /regulatory_class="terminator"
                     /note="Escherichia coli trpA"
     misc_feature    90..612
                     /note="partial sequence of Sinorhizobium meliloti nodB 
                     (Sma0868) and nodC (Sma0866)"
     misc_feature    624..635
                     /label=contains stop codons in all three reading frames
                     /note="contains stop codons in all three reading frames"
     gene            711..1278
                     /gene="tnpR"
                     /label=tnpR
     regulatory      711..717
                     /regulatory_class="ribosome_binding_site"
                     /gene="tnpR"
     CDS             727..1278
                     /codon_start=1
                     /gene="tnpR"
                     /product="gamma-delta resolvase"
                     /label=tnpR
                     /note="TnpR; site-specific recombinase; from pGOA1193"
                     /protein_id="ABX47153.1"
                     /translation="MRLFGYARVSTSQQSLDIQVRALKDAGVKANRIFTDKASGSSCDR
                     KGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVSIRFIDDGISTDGEMG
                     KMVVTILSAVAQAERQRILERTNEGRQEAMAKGVVFGRKRKIDRDAVLNMWQQGLGASH
                     ISKTMNIARSTVYKVINESN"
     gene            1278..3121
                     /gene="uidA"
                     /label=uidA
     regulatory      1278..1283
                     /regulatory_class="ribosome_binding_site"
                     /gene="uidA"
     CDS             1310..3121
                     /codon_start=1
                     /gene="uidA"
                     /product="beta-glucuronidase"
                     /label=uidA
                     /note="UidA; Gus; b-glucuronidase; from pVO155"
                     /protein_id="ABX47154.1"
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     CDS             3541..4335
                     /codon_start=1
                     /gene="nptII"
                     /product="neomycin phosphotransferase"
                     /label=nptII
                     /note="NptII; aminoglycoside-3-O-phosphotransferase; 
                     neomycin/kanamycin resistance; from pVO155"
                     /protein_id="ABX47155.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            3541..4335
                     /gene="nptII"
                     /label=nptII
     promoter        5345..5373
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             5421..6611
                     /codon_start=1
                     /gene="tetA"
                     /product="TetA"
                     /label=tetA
                     /note="tetracycline resistance"
                     /protein_id="ABX47156.1"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     gene            5421..6611
                     /gene="tetA"
                     /label=tetA
     promoter        7735..7763
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(8287..8303)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      8516..8971
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     oriT            9232..9553
     oriT            9310..9419
                     /note="incP origin of transfer"
     promoter        9589..9693
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9694..10554
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="Bla; b-lactamase; ampicillin resistance"
                     /protein_id="ABX47157.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            9694..10554
                     /gene="bla"
                     /label=bla
     rep_origin      10725..11313
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      10738..11364
                     /label=ColE1
                     /note="ColE1"