LOCUS       Exported                7327 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Gastrointestinal evaluation vector pVISIA11, complete sequence.
ACCESSION   KP892885
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Campos J, Mongodin EF, Fraser CM, Khan S.
  TITLE     In vivo gastrointestinal evaluation technology: barcoded molecular 
            tags to identify genetic determinants of E. coli colonization 
            efficiency in mice
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7327)
  AUTHORS   Huang S-H., Wu J, Cappiello C, Patel S, Hand N, Sridharan V, 
            Zeichner SL.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2015) Center for Cancer and Immunology Research, 
            Childrens National Medical Center, 111 Michigan Ave NW, Washington,
            DC 20010, USA
REFERENCE   3  (bases 1 to 7327)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7327
                     /organism="Gastrointestinal evaluation vector pVISIA11"
                     /lab_host="Escherichia coli str. K-12 substr. MG1655"
                     /mol_type="other DNA"
                     /db_xref="taxon:1777191"
     promoter        133..161
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             209..1399
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance protein"
                     /label=tetR
                     /note="antibiotic resistance to tetracycline"
                     /protein_id="AMB21819.1"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     gene            209..1399
                     /gene="tetR"
                     /label=tetR
     regulatory      1616..1805
                     /regulatory_class="promoter"
                     /note="bla promoter"
     promoter        1701..1805
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1806..3332
                     /codon_start=1
                     /product="AIDA-I autotransporter"
                     /label=AIDA-I autotransporter
                     /note="AIDA-I autotransporter with a modified signal 
                     sequence and passenger protein"
                     /protein_id="AMB21820.1"
                     /translation="MIKLKFGVFFTVLLSSAYAHGTPQNITHGVLTLSSRGLDYPYDVP
                     DYARYGRRVLTMEIFELNPTKESAGNTLTVSNYTGTPGSVISLGGVLEGDNSLTDRLVV
                     KGNTSGQSDIVYVNEDGSGGQTRDGINIISVEGNSDAEFSLKNRVVAGAYDYTLQKGNE
                     SGTDNKGWYLTSHLPTSDTRQYRPENGSYATNMALANSLFLMDLNERKQFRAMSDNTQP
                     ESASVWMKITGGISSGKLNDGQNKTTTNQFINQLGGDIYKFHAEQLGDFTLGIMGGYAN
                     AKGKTINYTSNKAARNTLDGYSVGVYGTWYQNGENATGLFAETWMQYNWFNASVKGDGL
                     EEEKYNLNGLTASAGGGYNLNVHTWTSPEGITGEFWLQPHLQAVWMGVTPDTHQEDNGT
                     VVQGAGKNNIQTKAGIRASWKVKSTLDKDTGRRFRPYIEANWIHNTHEFGVKMSDDSQL
                     LSGSRNQGEIKTGIEGVITQNLSVNGGVAYQAGGHGSNAISGALGIKYSF"
     misc_feature    1809..1886
                     /note="CTB signal sequence; Cholera toxin B signal 
                     sequence"
     misc_feature    1911..1955
                     /note="HA immunotag; hemagglutinin region recognized by 
                     commercial antibodies"
     CDS             1923..1949
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    1989..3332
                     /label=beta-barrel of the AIDA-I autotransporter
                     /note="beta-barrel of the AIDA-I autotransporter"
     CDS             3722..4516
                     /codon_start=1
                     /product="ThyA"
                     /label=ThyA
                     /note="E. coli thymidylate synthase"
                     /protein_id="AMB21822.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPLV
                     TTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWPTP
                     DGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLSCQL
                     YQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHLQLSR
                     EPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     misc_feature    4705..5364
                     /label=barcode region
                     /note="barcode region"
     rep_origin      complement(5400..6183)
                     /direction=LEFT
                     /label=pUC ori
                     /note="pUC ori"
     rep_origin      complement(5510..6098)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6289..7149)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="confers ampicillin resistance"
                     /protein_id="AMB21821.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     regulatory      complement(7150..7327)
                     /regulatory_class="promoter"
                     /note="bla promoter"
     promoter        complement(7150..7241)
                     /gene="bla"
                     /label=AmpR promoter