LOCUS       Exported                8808 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pVDHS1, complete sequence.
ACCESSION   KJ857553
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8808)
  AUTHORS   Vafadarnejad E, Amoozegar MA, Karimifard A.
  TITLE     Construction of an inducible expression vector for gene knock down 
            in Haloarchaeum Halobacterium salinarum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8808)
  AUTHORS   Vafadarnejad E, Deldar AA, Amoozegar MA, Bazzaz A, Karimifard A.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2014) Department of Microbiology, University of 
            Tehran, Qods Ave, Tehran 14155-6455, Iran
REFERENCE   3  (bases 1 to 8808)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8808
                     /organism="Expression vector pVDHS1"
                     /mol_type="other DNA"
                     /label=designed for Halobacterium salinarum
                     /note="designed for Halobacterium salinarum"
                     /db_xref="taxon:1538329"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      677..4426
     CDS             4824..6035
                     /codon_start=1
                     /gene="hmgA"
                     /product="HMG-CoA reductase"
                     /label=hmgA
                     /note="3-hydroxy-3-methylglutarylcoenzyme A reductase"
                     /protein_id="AIL23821.1"
                     /translation="MTDAASLADRVREGDLRLHELEAHADADTAAEARRLLVESQSGAS
                     LDAVGNYGFPAEAAESAIENMVGSIQVPMGVAGPVSVDGGSVAGEKYLPLATTEGALLA
                     SVNRGCSVINSAGGATARVLKSGMTRAPVFRVADVAEAEALVSWTRDNFAALKEAAEET
                     TNHGELLDVTPYVVGNSVYLRFRYDTKDAMGMNMATIATEAVCGVVEAETAASLVALSG
                     NLCSDKKPAAINAVEGRGRSVTADVRIPREVVEERLHTTPEAVAELNTRKNLVGSAKAA
                     SLGFNAHVANVVAAMFLATGQDEAQVVEGANAITTAEVQDGDLYVSVSIASLEVGTVGG
                     GTKLPTQSEGLDILGVSGGGDPAGSNADALAECIAVGSLAGELSLLSALASRHLSSAHA
                     ELGR"
     gene            4824..6035
                     /gene="hmgA"
                     /label=hmgA
     primer_bind     complement(6376..6392)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6620..6638)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6659..6675)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6683..6699
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6707..6737)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6752..6773
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7061..7649)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7820..8680)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8681..8785)
                     /gene="bla"
                     /label=AmpR promoter