LOCUS       Exported                5155 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Transformation and cloning vector pVCC, complete sequence.
ACCESSION   HQ207194
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Vannini A, Agriesti F, Mosca F, Roncarati D, Scarlato V, Danielli A.
  TITLE     A convenient and robust in vivo reporter system to monitor gene 
            expression in the human pathogen Helicobacter pylori
  JOURNAL   Appl. Environ. Microbiol. 78 (18), 6524-6533 (2012)
  PUBMED    22773640
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Danielli A.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-SEP-2010) Department of Biology, University of 
            Bologna, via Selmi, 3, Bologna, BO 40126, Italy
REFERENCE   3  (bases 1 to 5155)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Transformation and cloning vector pVCC"
                     /mol_type="other DNA"
                     /db_xref="taxon:983430"
     misc_recomb     1..564
                     /note="flanking region for double homologous recombination 
                     in Helicobacter pylori G27 vac::aphA-3/luxCDABE acceptor 
                     strain"
     CDS             27..542
                     /codon_start=1
                     /gene="cysS"
                     /product="CysS"
                     /label=cysS
                     /protein_id="ADZ38982.1"
                     /translation="YLLGVHYRSVLNFNEEDLLVSKKRLDKIYRLKQRVLGTLGGINPN
                     FKKEILECMQDDLNVSKALSVLESMLSSTNEKLDQNPKNKALKGEILANLKFIEELLGI
                     GFKDPSAYFQLGVSESEKQEIENKIEERKRAKEQKNFLKADSIREELLKQKIALMDTPQ
                     GTIWEKFF"
     gene            27..542
                     /gene="cysS"
                     /label=cysS
                     /note="Helicobacter pylori cysS cistron 5 end"
     CDS             complement(680..1303)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /protein_id="ADZ38980.1"
                     /translation="MQFTKIDINNWTRKEYFDHYFGNTPCTYSMTVKLDISKLKKDGKK
                     LYPTLLYGVTTIINRHEEFRTALDENGQVGVFSEMLPCYTVFHKETETFSSIWTEFTAD
                     YTEFLQNYQKDIDAFGERMGMSAKPNPPENTFPVSMIPWTSFEGFNLNLKKGYDYLLPI
                     FTFGKYYEEGGKYYIPLSIQVHHAVCDGFHVCRFLDELQDLLNK"
     gene            complement(680..1303)
                     /gene="cat"
                     /label=cat
                     /note="Campylobacter coli chloramphenicol resistance 
                     cassette"
     misc_feature    1407..1448
                     /note="MCS; multiple cloning site; unique BamHI, KpnI, 
                     SacI, and SnaBI cloning sites"
     gene            1440..2467
                     /gene="luxC"
                     /label=luxC
                     /note="Photorhabdus luminescens luxC cistron 5 end"
     regulatory      1440..1445
                     /regulatory_class="ribosome_binding_site"
                     /gene="luxC"
     misc_recomb     1451..2467
                     /note="flanking region for double homologous recombination 
                     in Helicobacter pylori G27 vac::aphA-3/luxCDABE acceptor 
                     strain"
     CDS             1451..2467
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC"
                     /label=luxC
                     /protein_id="ADZ38983.1"
                     /translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKES"
     promoter        complement(2476..2494)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2512..2528)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2536..2552
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2560..2590)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2605..2626
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      2896..3524
                     /label=pBR322 ori
                     /note="pBR322 ori"
     rep_origin      complement(2914..3502)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3673..4533)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="ADZ38981.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3673..4533)
                     /gene="bla"
                     /label=bla
                     /note="AmpR cassette"
     promoter        complement(4534..4638)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5112..5128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5135..5153
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"