LOCUS       Exported                6679 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pVC7N DNA, complete sequence.
ACCESSION   LC425431
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Hashiro S, Yasueda H.
  TITLE     High copy number mutants derived from Corynebacterium glutamicum 
            cryptic plasmid pAM330 and copy number control
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6679)
  AUTHORS   Hashiro S, Yasueda H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-SEP-2018) Contact:Shuhei Hashiro AJINOMOTO CO., INC., 
            Frontier Research Labs. Institute For Innovation; 1-1 Suzuki-Cho, 
            Kawasaki-Ku, Kawasaki, Kanagawa 210-8681, Japan URL 
            :http://www.ajinomoto.com/
REFERENCE   3  (bases 1 to 6679)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6679
                     /organism="Cloning vector pVC7N"
                     /mol_type="other DNA"
                     /note="pVC7N was constructed by ligating a cryptic plasmid 
                     of Corynebacterium glutamicum, pAM330N, to a vector for 
                     Escherichia Coli, pHSG399"
                     /db_xref="taxon:2382093"
     misc_feature    1..4451
                     /label=pAM330N region
                     /note="pAM330N region"
     CDS             1215..2600
                     /codon_start=1
                     /gene="repA"
                     /label=repA
                     /protein_id="BBG56731.1"
                     /translation="MTTIALAKTTGTTPAQTRTAQPRTTSGLGNTEIEVNTSKEPQVNE
                     GSKVTRARAWRRQNVMYKITNSKALAGCHRWRRDEAVAVSWSSNGASQFEGLQNSHSRW
                     GSPLAELEVMGERRIELAIATKNHLAAGGALMMFVGTVRHNRSQSFAQVEAGIKTAYSS
                     MVKTSQWKKERARYGVEHTYSDYEVTDSWANGWHLHRNMLLFLDRPLSDDELKAFEDSM
                     FSRWSAGVVKAGMDAPLREHGVKLDQVSTWGGDAAKMATYLAKGMSQELTGSATKTASK
                     GSYTPFQMLDMLADQSDAGEDMDAVLVARWREYEVGSKNLRSSWSRGAKRALGIDYIDA
                     DVRREMEEELYKLAGLEAPERVESTRVAVALVKPDDWKLIQSDFAVRQYVLDCVDKAKD
                     VAAAQRVANEVLASLGVDSTPCMIVMDDVDLDAVLPTHGDATKRDLNAAVFAGNEQTIL
                     RTH"
     gene            1215..2600
                     /gene="repA"
                     /label=repA
     misc_feature    4452..6679
                     /label=pHSG399 region
                     /note="pHSG399 region"
     CDS             4545..5204
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /protein_id="BBG56732.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            4545..5204
                     /gene="cat"
                     /label=cat
     protein_bind    5413..5434
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5449..5479
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5487..5503
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5511..5527
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5523..5753
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGE"
     misc_feature    5540..5596
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(5597..5613)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(5939..6527)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"