LOCUS       Exported                6742 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  RNAi cloning vector pVALIUM1, complete sequence.
ACCESSION   GU931383
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6742)
  AUTHORS   Ni J-Q., Liu L-P., Perkins LA, Perrimon N.
  TITLE     Vectors for transgenic flies
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6742)
  AUTHORS   Ni J-Q., Liu L-P., Perkins LA, Perrimon N.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2010) Genetics, Harvard Medical School, 77 Avenue 
            Louis Pasteur, Boston, MA 02115, USA
REFERENCE   3  (bases 1 to 6742)
  AUTHORS   Hu Y, Perkins LA.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2012) Genetics, Harvard Medical School, 77 Avenue 
            Louis Pasteur, Boston, MA 02115, USA
REFERENCE   4  (bases 1 to 6742)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On Jun 20, 2012 this sequence version replaced GU931383.1.
FEATURES             Location/Qualifiers
     source          1..6742
                     /organism="RNAi cloning vector pVALIUM1"
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
                     /db_xref="taxon:747299"
     rep_origin      complement(6..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    664..2543
                     /label=vermilion
                     /note="vermilion"
     CDS             complement(join(834..951,1028..1121,1213..1819,1874..2007,
                     2068..2228,2302..2327))
                     /codon_start=1
                     /product="vermillion"
                     /label=vermillion
                     /note="selectable marker"
                     /protein_id="ADE08345.1"
                     /translation="MSCPYAGNGNDHDDSAVPLTTEVGKIYGEYLMLDKLLDAQCMLSE
                     EDKRPVHDEHLFIITHQAYELWFKQIIFEFDSIRDMLDAEVIDETKTLEIVKRLNRVVL
                     ILKLLVDQVPILETMTPLDFMDFRKYLAPASGFQSLQFRLIENKLGVLTEQRVRYNQKY
                     SDVFSDEEARNSIRNSEKDPSLLELVQRWLERTPGLEESGFNFWAKFQESVDRFLEAQV
                     QSAMEEPVEKAKNYRLMDIEKRREVYRSIFDPAVHDALVRRGDRRFSHRALQGAIMITF
                     YRDEPRFSQPHQLLTLLMDIDSLITKWRYNHVIMVQRMIGSQQLGTGGSSGYQYLRSTL
                     SDRYKVFLDLFNLSTFLIPREAIPPLDETIRKKLINKSV"
     misc_recomb     2568..2935
                     /label=attB docking site
                     /note="attB docking site"
     protein_bind    2640..2709
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_recomb     2947..2980
                     /label=LoxP
                     /note="LoxP"
     protein_bind    complement(2947..2980)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    2987..3096
                     /label=5XUAS
                     /note="5XUAS"
     protein_bind    2993..3087
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     misc_recomb     3103..3136
                     /label=LoxP
                     /note="LoxP"
     protein_bind    complement(3103..3136)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    3143..3252
                     /label=5XUAS
                     /note="5XUAS"
     protein_bind    3149..3243
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     regulatory      3259..3518
                     /regulatory_class="promoter"
                     /note="Hsp70 promoter"
     promoter        3267..3505
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     misc_feature    3519..3524
                     /label=multiple cloning site
                     /note="multiple cloning site"
     intron          3547..3620
                     /note="white intron"
     misc_feature    3620..3625
                     /label=multiple cloning site
                     /note="multiple cloning site"
     intron          3669..3815
                     /note="ftz intron"
     misc_feature    3839..4538
                     /label=SV40 polyA region
                     /note="SV40 polyA region"
     intron          3915..3980
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4110..4130
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4402..4523
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(4557..4575)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4596..4612)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4620..4636
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4644..4674)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4689..4710
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4998..5586)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5757..6617)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    5760..6419
                     /label=AmpR
                     /note="AmpR"
     promoter        complement(6618..6722)
                     /gene="bla"
                     /label=AmpR promoter