LOCUS       Exported                7530 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pVAHS01it, complete sequence.
ACCESSION   KM925070
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   Vafadarnejad E, Amoozgar MA, Khansha J, Fallahzade R.
  TITLE     The rad2 gene of haloarchaeum Halobacterium salinarum is functional 
            in the repair of ultraviolet light induced DNA photoproducts
  JOURNAL   Microbiol. Res. 173, 44-49 (2015)
  PUBMED    25801970
REFERENCE   2  (bases 1 to 7530)
  AUTHORS   Vafadarnejad E, Amoozegar MA, Danaie Fard M, Khansha J.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2014) Department of Microbiology, University of 
            Tehran, Qods Ave., Tehran, Tehran 14155-6455, Iran
REFERENCE   3  (bases 1 to 7530)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7530
                     /organism="Cloning vector pVAHS01it"
                     /mol_type="other DNA"
                     /label=designed for Halobacterium salinarum strain R1
                     /note="designed for Halobacterium salinarum strain R1"
                     /db_xref="taxon:1618184"
     rep_origin      1..1183
     primer_bind     complement(1112..1128)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1136..1152
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1160..1190)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1205..1226
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1514..2102)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2273..3133)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3134..3238)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3710..3726
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4078..5997
                     /codon_start=1
                     /product="DNA gyrase subunit B"
                     /label=DNA gyrase subunit B
                     /protein_id="AJZ76765.1"
                     /translation="MSQDNEYGAGQIQVLEGLEAVRKRPAMYIGSTDSRGLHHLVYEVV
                     DNSIDEALAGHCDAIEVALHEDGSVSVTDNGRGIPVGTHEQYDRPALEVIMTVLHAGGK
                     FDNKSYQVSGGLHGVGVTVVNALSSELEVEVKHDGAVWTHRFEVGEPQVEEFERVRDLE
                     PGEDTGTTIRFWPDDGIFETTEFDFKTLENRLRELAFLNSGVEISLSDERTDESSTFLF
                     EGGIREFVEYLNETKTALHDDVIYYDDESEGIEVEIAMQATDELQGSIHAFANNINTRE
                     GGTHLTGFKTALTRVVNDYANSHDMLDDLDGDNLRGEDVREGLTAVISVKHPDPQFEGQ
                     TKTKLGNSEVRGIVESVTHQQLGTFFEENPDTATAIISKAVEAARARKAAKQAEELTRR
                     KSALESTSLPGKLADCQSRDPSESELFIVEGDSAGGSAKQGRDRKFQAILPLKGKILNV
                     EKHRLDRILENDEIRALITAIGGGVGDEFDIEKARYQRLILMTDADVDGAHIRTLLLTL
                     LYRHMRPLIEAGYVYAAQPPLYRVRYRGNTYDAMDEAERDRIIEEECNGNPTQVQRFKG
                     LGEMNPDQLWDTTMNPENRVLKRITVEDAAAADRMFNILMGDAVGPRKQFIKDHANDAE
                     WVDI"
     regulatory      6439..6550
                     /regulatory_class="promoter"
                     /gene="kdp"
     gene            6439..6550
                     /gene="kdp"
                     /label=kdp
     primer_bind     complement(6546..6562)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      6557..6777
                     /regulatory_class="terminator"