LOCUS       Exported                4423 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pV2, complete sequence.
ACCESSION   KJ569568
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Hertel R, Volland S, Liesegang H.
  TITLE     Conjugative reporter system for the use in Bacillus licheniformis 
            and closely related Bacilli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Hertel R, Volland S, Liesegang H.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2014) Genomic and Applied Microbiology, Goettingen
            Genomics Laboratory, Georg-August University Goettingen, 
            Grisebachstr.8, Goettingen, Lower Saxony D-37077, Germany
REFERENCE   3  (bases 1 to 4423)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Cloning vector pV2"
                     /mol_type="other DNA"
                     /db_xref="taxon:1499640"
     promoter        15..119
                     /gene="bla"
                     /label=AmpR promoter
     CDS             120..980
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="AHZ90955.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            120..980
                     /gene="bla"
                     /label=bla
     rep_origin      1151..1739
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      1154..1754
                     /label=oriBR322
                     /note="oriBR322"
     CDS             complement(2004..2774)
                     /codon_start=1
                     /gene="kan"
                     /product="kanamycin nucleotidyltransferase"
                     /label=kan
                     /protein_id="AHZ90956.1"
                     /translation="MRIVNGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLGR
                     QTDGPYSDIEMMCVMSTEEAEFSHEWTTGEWKVEVNFDSEEILLDYASQVESDWPLTHG
                     QFFSILPIYDSGGYLEKVYQTAKSVEAQTFHDAICALIVEELFEYAGKWRNIRVQGPTT
                     FLPSLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQSDLPSGYDHLCQFVMSGQLSD
                     SEKLLESLENFWNGIQEWTERHGYIVDVSKRIPF"
     gene            complement(2004..2774)
                     /gene="kan"
                     /label=kan
     oriT            complement(2971..3196)
                     /direction=LEFT
     oriT            3066..3175
                     /note="incP origin of transfer"
     CDS             complement(3203..4207)
                     /codon_start=1
                     /gene="repU"
                     /product="plasmid replication protein"
                     /label=repU
                     /protein_id="AHZ90957.1"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     gene            complement(3203..4207)
                     /gene="repU"
                     /label=repU
     rep_origin      complement(4359..4385)
                     /direction=LEFT
                     /label=oriU
                     /note="oriU"