LOCUS       Exported                7055 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Transposon delivery vector pUTkm1, complete sequence.
ACCESSION   AF102233
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7055)
  AUTHORS   de Lorenzo V, Herrero M, Jakubzik U, Timmis KN.
  TITLE     Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter 
            probing, and chromosomal insertion of cloned DNA in gram-negative 
            eubacteria
  JOURNAL   J. Bacteriol. 172 (11), 6568-6572 (1990)
  PUBMED    2172217
REFERENCE   2  (bases 1 to 7055)
  AUTHORS   Tombolini R, Jansson J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-1998) Department of Biochemistry, Stockholm 
            University, Svante Arrhenius vag. 10-12, Stockholm SE-10691, Sweden
REFERENCE   3  (bases 1 to 7055)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7055
                     /organism="Transposon delivery vector pUTkm1"
                     /mol_type="other DNA"
                     /db_xref="taxon:84477"
     CDS             complement(311..1171)
                     /codon_start=1
                     /gene="bla"
                     /product="beta lactamase"
                     /label=bla
                     /protein_id="AAC79082.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(311..1171)
                     /gene="bla"
                     /label=bla
     promoter        complement(1172..1276)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    1321..2835
                     /label=originates from Escherichia coli transposon Tn5
                     /note="originates from Escherichia coli transposon Tn5"
     CDS             3394..4209
                     /codon_start=1
                     /product="kanamycin resistance protein"
                     /label=kanamycin resistance protein
                     /note="originates from transposon Tn903"
                     /protein_id="AAC79083.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     protein_bind    4718..4734
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     rep_origin      4769..5157
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(5775..6146)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6179..6288
                     /note="incP origin of transfer"