LOCUS       Exported                6826 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pUT1 DNA, complete sequence.
ACCESSION   LC016841
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Kasai Y, Oshima K, Ikeda F, Abe J, Yoshimitsu Y, Harayama S.
  TITLE     Construction of self-cloning system in a unicellular green algae, 
            Pseudochoricystis ellipsoidea
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6826)
  AUTHORS   Kasai Y, Oshima K, Ikeda F, Abe J, Yoshimitsu Y, Harayama S.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2014) Contact:Yuki Kasai Chuo University, 
            Biological Science; 1-13-27 Kasuga, Bunkyo-ku, Tokyo 112-8551, Japan
REFERENCE   3  (bases 1 to 6826)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Expression vector pUT1"
                     /mol_type="other DNA"
                     /db_xref="taxon:1589842"
     regulatory      5..960
                     /regulatory_class="promoter"
                     /note="includes promoter region of Pseudochoricystis 
                     ellipsoidea beta-tubulin (TUBULIN1) gene"
     CDS             963..2465
                     /codon_start=1
                     /gene="UMPS"
                     /product="uridine monophosphate synthase"
                     /label=UMPS
                     /protein_id="BAR90701.1"
                     /translation="MATSTPSVATAAELKPKEGPITSQEFEELVLRLHEIEAVKFGNFK
                     LKSGLMSPIYIDLRVIVSYPDVLRRVAEVMWHQVSGAAFDVMCGVPYTALPIATCMSLL
                     HGTPMLMRRKEVKEYGTKKAIEGAFKKGQTCLIVEDLVTSGASVMETVEPLEVEGLKVT
                     DVVVLIDREQGGAARMASNGLRLHSAFTLSFIIKTLQAHGLVSAKVADSVAAFIAANQT
                     FTPSAAAPTPSPAAPAQPKRLPFEERASLCQNAAGRKLLELMARKRTNLAVAADVATVE
                     EMLRIADAAGPHIAVFKTHVDIFDKWDDGIATQLRHLADKHEFLIFEDRKFADIGNTVV
                     SQYGGGIYKIADWSDITNAHLVPGPGIIDGLRKVGQEKGRGLLLLAEMSSKGALATGAY
                     TEKVAEAAAANQDFVMGFICQSPAKWATPVPPGLVHMTPGVQLASGSDALGQQYNTPAS
                     VIGQGGSDVIIVGRGIIKAADPAAAAAQYREAGWAAYEATLA"
     gene            963..2465
                     /gene="UMPS"
                     /label=UMPS
     regulatory      2463..3898
                     /regulatory_class="terminator"
                     /note="includes terminator region of Pseudochoricystis 
                     ellipsoidea actin (ACTIN1) gene"
     misc_feature    3899..6826
                     /note="derived from plasmid pBluescript SK(+), Acc#: 
                     X52328.1"
     promoter        complement(3920..3938)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3959..3975)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3983..3999
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4007..4037)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4052..4073
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4361..4949)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5120..5980)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5981..6085)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6112..6567
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     6708..6724
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6734..6752
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     6785..6801
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"