LOCUS       Exported                5453 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pURYL, complete sequence.
ACCESSION   JQ825243
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Lv H, Gong Z.
  TITLE     Cost-effective and repeatable gene targeting in fission yeast using 
            a cloning-based and modular method
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Lv H, Gong Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2012) School of Life Science, Fudan University, 
            Room 618, Science Building, Handan Road 220, Shanghai 200433, China
REFERENCE   3  (bases 1 to 5453)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Cloning vector pURYL"
                     /mol_type="other DNA"
                     /note="cloning vector for C-terminal mYFP-6xHis taging in 
                     fission yeast"
                     /db_xref="taxon:1276088"
     promoter        235..253
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    292..1002
                     /label=mYFP
                     /note="mYFP"
     misc_feature    1003..1020
                     /label=6xHis tag
                     /note="6xHis tag"
     CDS             1003..1020
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      1030..1522
                     /regulatory_class="terminator"
                     /note="Saccharomyces cerevisiae Leu2 terminator"
     regulatory      1529..2050
                     /regulatory_class="promoter"
                     /note="Schizosaccharomyces pombe Ura4 promoter"
     CDS             2051..2845
                     /codon_start=1
                     /product="URA4"
                     /label=URA4
                     /note="Schizosaccharomyces pombe Ura4 (Orotidine 
                     5-phosphate decarboxylase)"
                     /protein_id="AGB50987.1"
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     regulatory      2846..3275
                     /regulatory_class="terminator"
                     /note="Schizosaccharomyces pombe Ura4 terminator"
     promoter        complement(3342..3360)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3603..4222
                     /label=pBR322 origin
                     /note="pBR322 origin"
     rep_origin      complement(3618..4206)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4377..5237)
                     /codon_start=1
                     /product="AmpR"
                     /label=AmpR
                     /note="beta-lactamase"
                     /protein_id="AGB50988.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5238..5342)
                     /gene="bla"
                     /label=AmpR promoter