LOCUS       Exported                5653 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pUNIV, complete sequence.
ACCESSION   EF177812
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Venkatachalan SP, Bushman JD, Mercado JL, Sancar F, Christopherson 
            KR, Boileau AJ.
  TITLE     Optimized expression vector for ion channel studies in Xenopus 
            oocytes and mammalian cells using alfalfa mosaic virus
  JOURNAL   Pflugers Arch. 454 (1), 155-163 (2007)
  PUBMED    17146677
REFERENCE   2  (bases 1 to 5653)
  AUTHORS   Boileau AJ, Venkatachalan SP, Bushman JD, Mercado JL.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2006) Physiology, Univ. of Wisconsin-Madison, 601 
            Science Dr., Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 5653)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Expression vector pUNIV"
                     /mol_type="other DNA"
                     /note="AMV sequence is included in the 5 oligonucleotide 
                     used as primer for the insert of interest"
                     /db_xref="taxon:419107"
     regulatory      1..659
                     /regulatory_class="enhancer"
                     /note="Cytomegalovirus immediate-early enhancer"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      669..750
                     /regulatory_class="promoter"
                     /note="Cytomegalovirus immediate-early promoter"
     intron          890..1022
                     /note="chimeric intron"
     regulatory      1067..1085
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1085..1107
                     /label=modified multiple cloning region
                     /note="modified multiple cloning region"
     misc_feature    1108..1306
                     /label=Xenopus laevis beta-globin 3 flanking region
                     /note="Xenopus laevis beta-globin 3 flanking region"
     3UTR           1124..1249
                     /label=Xenopus globin 3-UTR
                     /note="3-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     regulatory      complement(1321..1339)
                     /regulatory_class="promoter"
                     /note="T3 promoter"
     promoter        complement(1322..1339)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      1348..1569
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 late polyadenylation signal"
     polyA_signal    1357..1478
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      1664..2119
                     /label=f1 ori
                     /note="f1 ori"
     misc_feature    2181..2599
                     /label=SV40 enhancer and early promoter for neo-r gene
                     /note="SV40 enhancer and early promoter for neo-r gene"
     promoter        2236..2593
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2444..2579
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    2497..2562
                     /label=SV40 minimum origin of replication
                     /note="SV40 minimum origin of replication"
     CDS             2644..3438
                     /codon_start=1
                     /product="neomycin phosphotransferase"
                     /label=neomycin phosphotransferase
                     /protein_id="ABM65100.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    3502..3550
                     /note="synthetic polyadenylation signal for neomycin 
                     phosphotransferase"
     polyA_signal    3502..3550
                     /note="synthetic polyadenylation signal"
     promoter        3856..3960
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3961..4821
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="ABM65099.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4992..5580
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"