LOCUS       Exported                3090 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUK21, complete sequence.
ACCESSION   AF223640
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Vieira J, Messing J.
  TITLE     New pUC-derived cloning vectors with different selectable markers 
            and DNA replication origins
  JOURNAL   Gene 100, 189-194 (1991)
  PUBMED    1905257
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Vieira J, Messing J.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2000) Waksman Institute, Rutgers University, 190 
            Frelinghuysen Road, Piscataway, NJ 08854, USA
REFERENCE   3  (bases 1 to 3090)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Cloning vector pUK21"
                     /lab_host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:119973"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    183..199
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     207..223
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             219..659
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPLVRGLEIYRCMPWYPGARIRSFCRRVDVIWIRYRRGGRS
                     RTSGSIPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRP
                     SQQLRSLNGEWRLMRYFLLTHLCGISHRIRQSNHSTRPVAAH"
     primer_bind     351..367
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(382..400)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(407..423)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1360..2178
                     /codon_start=1
                     /gene="kanR"
                     /product="aminoglycoside 3-phosphotransferase"
                     /label=kanR
                     /note="KanR"
                     /protein_id="AAF71713.1"
                     /translation="MSHIQRETSCSTRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPG
                     KTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
                     ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVG
                     IADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            1360..2178
                     /gene="kanR"
                     /label=kanR
     rep_origin      2321..2909
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"