LOCUS       Exported                8887 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pUH-EX DNA, complete sequence.
ACCESSION   AB084895
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8887)
  AUTHORS   Onizuka T, Akiyama H, Umeda Y, Hirano A, Ikeuchi M.
  TITLE     Expression vector pUH-EX for cyanobacteria
  JOURNAL   Published Only in Database (2002)
REFERENCE   2  (bases 1 to 8887)
  AUTHORS   Onizuka T, Akiyama H, Umeda Y, Hirano A, Ishibashi M, Ikeuchi M, 
            Inoue Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2002) Takuo Onizuka, Toray Reserch Center, Inc., 
            Biological Sciences Department; Tebiro 1111, Kamakura, Kanagawa 
            248-8555, Japan (E-mail:Takuo_Onizuka@trc.toray.co.jp, 
            Tel:81-467-32-9965, Fax:81-467-32-0414)
REFERENCE   3  (bases 1 to 8887)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8887
                     /organism="Expression vector pUH-EX"
                     /mol_type="other DNA"
                     /note="derived from puc18 and pUH24, expression vector for 
                     cyanobacteria"
                     /db_xref="taxon:194967"
     promoter        complement(147..175)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     misc_feature    250..255
                     /label=XbaI cloning site
                     /note="XbaI cloning site"
     misc_feature    256..261
                     /label=BamHI cloning site
                     /note="BamHI cloning site"
     misc_feature    261..266
                     /label=SmaI cloning site
                     /note="SmaI cloning site"
     misc_feature    271..276
                     /label=SacI cloning site
                     /note="SacI cloning site"
     misc_feature    277..282
                     /label=EcoRI cloning site
                     /note="EcoRI cloning site"
     primer_bind     complement(292..308)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    316..332
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(340..370)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    385..406
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(694..1282)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1453..2313)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2314..2418)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2892..2908
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      6756..8557
                     /regulatory_class="promoter"
                     /note="Promoter of the phycocyanin gene of the 
                     cyanobacterium Synechococcus sp. PCC7942"
     misc_feature    8556..8561
                     /label=NcoI cloning site
                     /note="NcoI cloning site"
     misc_feature    8882..8887
                     /label=EcoRV cloning site
                     /note="EcoRV cloning site"