LOCUS       Exported                6697 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pUDI069, complete sequence.
ACCESSION   JX268036
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6697)
  AUTHORS   Koopman F, Beekwilder J, Crimi B, Houwelingen A, Hall RD, Bosch D, 
            van Maris AJ, Pronk JT, Daran JM.
  TITLE     De novo production of the flavonoid naringenin in engineered 
            Saccharomyces cerevisiae
  JOURNAL   Microb. Cell Fact. 11 (1), 155 (2012)
  PUBMED    23216753
REFERENCE   2  (bases 1 to 6697)
  AUTHORS   Koopman F, Beekwilder J, Crimi B, van Houwelingen A, Hall RD, Bosch 
            D, Maris AJA., Pronk JT, Daran J-M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2012) Platform Green Synthetic Biolog, Delft 2600,
            The Netherlands
REFERENCE   3  (bases 1 to 6697)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6697
                     /organism="Expression vector pUDI069"
                     /lab_host="Saccharomyces cerevisiae"
                     /mol_type="other DNA"
                     /db_xref="taxon:1269196"
     rep_origin      133..752
                     /label=Coli origin of replication
                     /note="Coli origin of replication"
     rep_origin      complement(148..736)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(907..1767)
                     /codon_start=1
                     /product="ampicilin resistance"
                     /label=ampicilin resistance
                     /note="bla"
                     /protein_id="AGA15804.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1768..1872)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2152..2432
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             2433..3107
                     /codon_start=1
                     /product="auxotrophic marker"
                     /label=auxotrophic marker
                     /note="TRP1"
                     /protein_id="AGA15803.1"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(3204..3659)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     3804..3820
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3827..3845
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        3870..4514
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase"
     regulatory      3987..4526
                     /regulatory_class="promoter"
                     /note="TDH3 promoter"
     CDS             4532..6127
                     /codon_start=1
                     /product="tyrosine ammonia lyase"
                     /label=tyrosine ammonia lyase
                     /note="Tal1"
                     /protein_id="AGA15802.1"
                     /translation="MTLQSQTAKDCLALDGALTLVQCEAIATHRSRISVTPALRERCAR
                     AHARLEHAIAEQRHIYGITTGFGPLANRLIGADQGAELQQNLIYHLATGVGPKLSWAEA
                     RALMLARLNSILQGASGASPETIDRIVAVLNAGFAPEVPAQGTVGASGDLTPLAHMVLA
                     LQGRGRMIDPSGRVQEAGAVMDRLCGGPLTLAARDGLALVNGTSAMTAIAALTGVEAAR
                     AIDAALRHSAVLMEVLSGHAEAWHPAFAELRPHPGQLRATERLAQALDGAGRVCRTLTA
                     ARRLTAADLRPEDHPAQDAYSLRVVPQLVGAVWDTLDWHDRVVTCELNSVTDNPIFPEG
                     CAVPALHGGNFMGVHVALASDALNAALVTLAGLVERQIARLTDEKLNKGLPAFLHGGQA
                     GLQSGFMGAQVTATALLAEMRANATPVSVQSLSTNGANQDVVSMGTIAARRARAQLLPL
                     SQIQAILALALAQAMDLLDDPEGQAGWSLTARDLRDRIRAVSPGLRADRPLAGHIEAVA
                     QGLRHPSAAADPPA"
     terminator      6134..6381
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     regulatory      6141..6380
                     /regulatory_class="terminator"
                     /operon="CYC1 terminator"
     promoter        complement(6404..6422)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6443..6459)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6467..6483
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6491..6521)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"