LOCUS       Exported               12865 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pUDE172, complete sequence.
ACCESSION   JX268037
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12865)
  AUTHORS   Koopman F, Beekwilder J, Crimi B, Houwelingen A, Hall RD, Bosch D, 
            van Maris AJ, Pronk JT, Daran JM.
  TITLE     De novo production of the flavonoid naringenin in engineered 
            Saccharomyces cerevisiae
  JOURNAL   Microb. Cell Fact. 11 (1), 155 (2012)
  PUBMED    23216753
REFERENCE   2  (bases 1 to 12865)
  AUTHORS   Koopman F, Beekwilder J, Crimi B, van Houwelingen A, Hall RD, Bosch 
            D, Maris AJA., Pronk JT, Daran J-M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2012) Platform Green Synthetic Biolog, Delft 2600,
            The Netherlands
REFERENCE   3  (bases 1 to 12865)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12865
                     /organism="Expression vector pUDE172"
                     /lab_host="Saccharomyces cerevisiae"
                     /mol_type="other DNA"
                     /db_xref="taxon:1269197"
     regulatory      2..656
                     /regulatory_class="promoter"
                     /note="TDH3 promoter"
     promoter        8..651
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase"
     CDS             657..2834
                     /codon_start=1
                     /product="PAL1"
                     /label=PAL1
                     /protein_id="AGA15805.1"
                     /translation="MEINGAHKSNGGGVDAMLCGGDIKTKNMVINAEDPLNWGAAAEQM
                     KGSHLDEVKRMVAEFRKPVVNLGGETLTIGQVAAISTIGNSVKVELSETARAGVNASSD
                     WVMESMNKGTDSYGVTTGFGATSHRRTKNGVALQKELIRFLNAGIFGSTKETSHTLPHS
                     ATRAAMLVRINTLLQGFSGIRFEILEAITSFLNNNITPSLPLRGTITASGDLVPLSYIA
                     GLLTGRPNSKATGPNGEALTAEEAFKLAGISSGFFDLQPKEGLALVNGTAVGSGMASMV
                     LFETNVLSVLAEILSAVFAEVMSGKPEFTDHLTHRLKHHPGQIEAAAIMEHILDGSSYM
                     KLAQKLHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRYATKSIEREINSVNDNPLIDVS
                     RNKAIHGGNFQGTPIGVSMDNTRLAIAAIGKLMFAQFSELVNDFYNNGLPSNLTASRNP
                     SLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTSEAVDIL
                     KLMSTTFLVAICQAVDLRHLEENLRQTVKNTVSQVAKKVLTTGVNGELHPSRFCEKDLL
                     KVVDREQVYTYADDPCSATYPLIQKLRQVIVDHALINGESEKNAVTSIFHKIGAFEEEL
                     KAVLPKEVEAARAAYDNGTSAIPNRIKECRSYPLYRFVREELGTELLTGEKVTSPGEEF
                     DKVFTAICEGKIIDPMMECLNEWNGAPIPIC"
     regulatory      2835..3086
                     /regulatory_class="terminator"
                     /operon="CYC1 terminator"
     terminator      2835..3082
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     misc_feature    3090..3169
                     /label=recombination site B
                     /note="recombination site B"
     regulatory      3170..4076
                     /regulatory_class="promoter"
                     /note="TPI1 promoter"
     CDS             4078..5595
                     /codon_start=1
                     /product="C4H"
                     /label=C4H
                     /protein_id="AGA15806.1"
                     /translation="MDLLLLEKSLIAVFVAVILATVISKLRGKKLKLPPGPIPIPIFGN
                     WLQVGDDLNHRNLVDYAKKFGDLFLLRMGQRNLVVVSSPDLTKEVLLTQGVEFGSRTRN
                     VVFDIFTGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQNREGWEFEAASVVEDVKK
                     NPDSATKGIVLRKRLQLMMYNNMFRIMFDRRFESEDDPLFLRLKALNGERSRLAQSFEY
                     NYGDFIPILRPFLRGYLKICQDVKDRRIALFKKYFVDERKQIASSKPTGSEGLKCAIDH
                     ILEAEQKGEINEDNVLYIVENINVAAIETTLWSIEWGIAELVNHPEIQSKLRNELDTVL
                     GPGVQVTEPDLHKLPYLQAVVKETLRLRMAIPLLVPHMNLHDAKLAGYDIPAESKILVN
                     AWWLANNPNSWKKPEEFRPERFFEEESHVEANGNDFRYVPFGVGRRSCPGIILALPILG
                     ITIGRMVQNFELLPPPGQSKVDTSEKGGQFSLHILNHSIIVMKPRNC"
     regulatory      5596..6059
                     /regulatory_class="terminator"
                     /note="ADH1 terminator"
     terminator      5734..5921
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     misc_feature    6063..6142
                     /label=recombination site C
                     /note="recombination site C"
     regulatory      6146..6520
                     /regulatory_class="promoter"
                     /note="PGI1 promoter"
     CDS             6527..8605
                     /codon_start=1
                     /product="CPR1"
                     /label=CPR1
                     /protein_id="AGA15807.1"
                     /translation="MTSALYASDLFKQLKSIMGTDSLSDDVVLVIATTSLALVAGFVVL
                     LWKKTTADRSGELKPLMIPKSLMAKDEDDDLDLGSGKTRVSIFFGTQTGTAEGFAKALS
                     EEIKARYEKAAVKVIDLDDYAADDDQYEEKLKKETLAFFCVATYGDGEPTDNAARFYKW
                     FTEENERDIKLQQLAYGVFALGNRQYEHFNKIGIVLDEELCKKGAKRLIEVGLGDDDQS
                     IEDDFNAWKESLWSELDKLLKDEDDKSVATPYTAVIPEYRVVTHDPRFTTQKSMESNVA
                     NGNTTIDIHHPCRVDVAVQKELHTHESDRSCIHLEFDISRTGITYETGDHVGVYAENHV
                     EIVEEAGKLLGHSLDLVFSIHADKEDGSPLESAVPPPFPGPCTLGTGLARYADLLNPPR
                     KSALVALAAYATEPSEAEKLKHLTSPDGKDEYSQWIVASQRSLLEVMAAFPSAKPPLGV
                     FFAAIAPRLQPRYYSISSSPRLAPSRVHVTSALVYGPTPTGRIHKGVCSTWMKNAVPAE
                     KSHECSGAPIFIRASNFKLPSNPSTPIVMVGPGTGLAPFRGFLQERMALKEDGEELGSS
                     LLFFGCRNRQMDFIYEDELNNFVDQGVISELIMAFSREGAQKEYVQHKMMEKAAQVWDL
                     IKEEGYLYVCGDAKGMARDVHRTLHTIVQEQEGVSSSEAEAIVKKLQTEGRYLRDVW"
     regulatory      8611..8877
                     /regulatory_class="terminator"
                     /operon="PGI1 terminator"
     misc_feature    8917..8996
                     /label=recombination site D
                     /note="recombination site D"
     CDS             complement(9126..9929)
                     /codon_start=1
                     /product="uracil auxotrophic marker"
                     /label=uracil auxotrophic marker
                     /note="URA3"
                     /protein_id="AGA15809.1"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(9930..10145)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     misc_feature    10415..10918
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     rep_origin      10438..10918
                     /label=CEN6+ARSH4
                     /note="CEN6+ARSH4"
     promoter        10955..11059
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11060..11920
                     /codon_start=1
                     /product="ampicilin resistance"
                     /label=ampicilin resistance
                     /note="bla"
                     /protein_id="AGA15808.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      12091..12679
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    12784..12863
                     /label=recombination site A
                     /note="recombination site A"