LOCUS       Exported               10005 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUDC082, complete sequence.
ACCESSION   KF680164
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10005)
  AUTHORS   Beekwilder J, van Rossum H.
  TITLE     Production of carotenoids in Saccharomyces cerevisiae by a 
            self-processing polyprotein and conversion to beta-ionone
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10005)
  AUTHORS   Beekwilder J, van Rossum H.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2013) BU Bioscience, Plant Research International,
            P.O.Box 619, Wageningen, Gelderland 6700AP, Netherlands
REFERENCE   3  (bases 1 to 10005)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: IDBA v. 1
            Coverage              :: 160
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10005
                     /organism="Cloning vector pUDC082"
                     /mol_type="other DNA"
                     /db_xref="taxon:1497751"
     regulatory      1..655
                     /regulatory_class="promoter"
                     /note="GPD promoter"
     promoter        7..650
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase"
     primer_bind     656..672
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             674..5683
                     /codon_start=1
                     /product="fusion protein"
                     /label=fusion protein
                     /protein_id="AHZ97961.1"
                     /translation="MTALAYYQIHLIYTLPILGLLGLLTSPILTKFDIYKISILVFIAF
                     SATTPWDSWIIRNGAWTYPSAESGQGVFGTFLDVPYEEYAFFVIQTVITGLVYVLATRH
                     LLPSLALPKTRSSALSLALKALIPLPIIYLFTAHPSPSPDPLVTDHYFYMRALSLLITP
                     PTMLLAALSGEYAFDWKSGRAKSTIATIMIPTVYLIWVDYVAVGQDSWSINDEKIVGWR
                     LGGVLPIEEAMFFLLTNLMIVLGLSACDHTQALYLLHGRTIYGNKKMPSSFPLITPPVL
                     SLFFSSRPYSSQPKRDLELAVKLLEKKSRSFFVASAGFPSEVRERLVGLYAFCRVTDDL
                     IDSPEVSSNPHATIDMVSDFLTLLFGPPLHPSQPDKILSSPLLPPSHPSRPTGMYPLPP
                     PPSLSPAELVQFLTERVPVQYHFAFRLLAKLQGLIPRYPLDELLRGYTTDLIFPLSTEA
                     VQARKTPIETTADLLDYGLCVAGSVAELLVYVSWASAPSQVPATIEEREAVLVASREMG
                     TALQLVNIARDIKGDATEGRFYLPLSFFGLRDESKLAIPTDWTEPRPQDFDKLLSLSPS
                     STLPSSNASESFRFEWKTYSLPLVAYAEDLAKHSYKGIDRLPTEVQAGMRAACASYLLI
                     GREIKVVWKGDVGERRTVAGWRRVRKVLSVVMSGWEGQRAEGRGSLLTCGDVEENPGPG
                     KEQDQDKPTAIIVGCGIGGIATAARLAKEGFQVTVFEKNDYSGGRCSLIERDGYRFDQG
                     PSLLLLPDLFKQTFEDLGEKMEDWVDLIKCEPNYVCHFHDEETFTFSTDMALLKREVER
                     FEGKDGFDRFLSFIQEAHRHYELAVVHVLQKNFPGFAAFLRLQFIGQILALHPFESIWT
                     RVCRYFKTDRLRRVFSFAVMYMGQSPYSAPGTYSLLQYTELTEGIWYPRGGFWQVPNTL
                     LQIVKRNNPSAKFNFNAPVSQVLLSPAKDRATGVRLESGEEHHADVVIVNADLVYASEH
                     LIPDDARNKIGQLGEVKRSWWADLVGGKKLKGSCSSLSFYWSMDRIVDGLGGHNIFLAE
                     DFKGSFDTIFEELGLPADPSFYVNVPSRIDPSAAPEGKDAIVILVPCGHIDASNPQDYN
                     KLVARARKFVIQTLSAKLGLPDFEKMIVAEKVHDAPSWEKEFNLKDGSILGLAHNFMQV
                     LGFRPSTRHPKYDKLFFVGASTHPGTGVPIVLAGAKLTANQVLESFDRSPAPDPNMSLS
                     VPYGKPLKSNGTGIDSQVQLKFMDLERWVYLLVLLIGAVIARSVGVLAFRAEGRGSLLT
                     CGDVEENPGPDYANILTAIPLEFTPQDDIVLLEPYHYLGKNPGKEIRSQLIEAFNYWLD
                     VKKEDLEVIQNVVGMLHTASLLMDDVEDSSVLRRGSPVAHLIYGIPQTINTANYVYFLA
                     YQEIFKLRPTPIPMPVIPPSSASLQSSVSSASSSSSASSENGGTSTPNSQIPFSKDTYL
                     DKVITDEILSLHRGQGLELFWRDSLTCPSEEEYVKMVLGKTGGLFRIAVRLMMAKSECD
                     IDFVQLVNLISIYFQIRDDYMNLQSSEYAHNKNFAEDLTEGKFSFPTIHSIHANPSSRL
                     VINTLQKKSTSPEILHHCVNYMRTETHSFEYTQEVLNTLSGALERELGRLQGEFAEANS
                     RMDLGDVDSEGRTGKNVKLEAILKKLADIPL"
     misc_feature    674..2692
                     /note="bifunctional protein lycopene cyclase and phytoene 
                     synthase from Xanthophyllomyces dendrorhous; Region: CrtYB"
     misc_feature    2693..2752
                     /label=Region: T2A ribosomal skipping sequence
                     /note="Region: T2A ribosomal skipping sequence"
     CDS             2699..2752
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    2753..4495
                     /note="phytoene desaturase from Xanthophyllomyces 
                     dendrorhous; Region: CrtI"
     misc_feature    4496..4555
                     /label=Region: T2A ribosomal skipping sequence
                     /note="Region: T2A ribosomal skipping sequence"
     CDS             4502..4555
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    4556..5680
                     /note="GGPP synthase from Xanthophyllomyces dendrorhous; 
                     Region: CrtE"
     CDS             5091..5102
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      5748..5945
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     regulatory      5751..5964
                     /regulatory_class="terminator"
                     /note="TEF1"
     CDS             6439..7242
                     /codon_start=1
                     /product="orotidine-5-phosphate decarboxylase"
                     /label=orotidine-5-phosphate decarboxylase
                     /note="URA3; derived from Kluyveromyces lactis"
                     /protein_id="AHZ97959.1"
                     /translation="MSTKSYTSRAETHASPVASKLLRLMDEKKTNLCASLDVRSTDELL
                     KLVETLGPYICLLKTHVDILDDFSYEGTVVPLKALAEKYKFLIFEDRKFADIGNTVKLQ
                     YTSGVYRIAEWSDITNAHGVTGAGIVAGLKQGAQEVTKEPRGLLMLAELSSKGSLAHGE
                     YTKGTVDIAKSDKDFVIGFIAQNDMGGREEGFDWLIMTPGVGLDDKGDALGQQYRTVDE
                     VVSGGSDIIIVGRGLFAKGRDPKVEGERYRNAGWEAYQKRISAPH"
     promoter        7426..7530
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7531..8391
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="AHZ97960.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8562..9150
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    9422..9925
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     rep_origin      9445..9808
     rep_origin      9809..9925