LOCUS       Exported                4818 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pUCYIT356N DNA, complete sequence.
ACCESSION   AB119527
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Kushiro A, Kiwaki M, Shimizu K.
  TITLE     A cryptic plasmid of Lactobacillus casei and construction of shuttle
            vector and food-grade vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Kushiro A, Kiwaki M, Shimizu K.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2003) Akira Kushiro, Yakult Central Institute for 
            Microbiological Research, Clinical Microbiology; 1796, Yaho, 
            Kunitachi, Tokyo 186-8650, Japan (E-mail:akira-kushiro@yakult.co.jp,
            Tel:81-42-577-8962(ex.2403), Fax:81-42-577-3020)
REFERENCE   3  (bases 1 to 4818)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Shuttle vector pUCYIT356N"
                     /focus
                     /mol_type="other DNA"
                     /db_xref="taxon:244934"
     source          1378..3697
                     /organism="Lactobacillus casei"
                     /plasmid="pYIT356"
                     /mol_type="other DNA"
                     /db_xref="taxon:1582"
     regulatory      176..181
                     /regulatory_class="minus_35_signal"
     regulatory      203..208
                     /regulatory_class="minus_10_signal"
     CDS             264..1001
                     /codon_start=1
                     /gene="emr"
                     /product="erythromycin resistance gene"
                     /label=emr
                     /protein_id="BAD42832.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTT
                     KLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYH
                     LSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFH
                     PKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNN
                     LSTVTYEQVLSIFNSYLLFNGRK"
     gene            264..1001
                     /gene="emr"
                     /label=emr
     primer_bind     1308..1324
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   2056..2076
                     /label=DR1
                     /note="DR1"
     repeat_region   2065..2080
     repeat_region   2077..2097
                     /label=DR2
                     /note="DR2"
     repeat_region   2086..2101
     repeat_region   2098..2118
                     /label=DR3
                     /note="DR3"
     repeat_region   2107..2122
     repeat_region   2119..2139
                     /label=DR4
                     /note="DR4"
     repeat_region   2128..2143
                     /rpt_unit_range=2128 .. 2143
     regulatory      2152..2157
                     /regulatory_class="minus_35_signal"
                     /inference="non-experimental evidence, no additional
                     details recorded"
     regulatory      2175..2180
                     /regulatory_class="minus_10_signal"
                     /inference="non-experimental evidence, no additional
                     details recorded"
     CDS             2224..3036
                     /codon_start=1
                     /gene="repB"
                     /product="Replication protein"
                     /label=repB
                     /experiment="experimental evidence, no additional details 
                     recorded"
                     /protein_id="BAD42833.1"
                     /translation="MNVALAGLLDRQDYLVTQANDLARSLGNLSTFQHKVLDYCFSYVT
                     ANDNANTVYHLTTLEIIHHLGLNTSGDSYKRVVKAFKALNENTAIYMRTTEPDGKHGIL
                     MTSLFAYIKVIDDGRVEFKFSATVAPFVFQLKRQFYSFHLSELTRVRSKYTLSMMKLWN
                     ANAIGKWRDQNDPSSLPPAATIKGSLTDWESWFLGSDDDGKPIKWPAGRFKQKAIDVAI
                     KELGSLYPQTMINVLRLTHGRRIEGFQIEFRPIRTVLDLNPQVIDGKP"
     gene            2224..3036
                     /gene="repB"
                     /label=repB
     primer_bind     complement(3718..3734)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3742..3758
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3766..3796)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3811..3832
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4120..4708)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"