LOCUS       Exported                4453 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Transient gene expression vector pUCPMA-M24, complete sequence.
ACCESSION   KT388099
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Maiti IB, Dey N.
  TITLE     Further characterization and expression analysis of Mirabilis mosaic
            caulimovirus (MMV) full-length transcript promoter with single and 
            double enhancer domains in transgenic plants
  JOURNAL   Transgenics 3, 61-70 (1999)
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Dey N, Maiti IB.
  TITLE     Structure and promoter/leader deletion analysis of mirabilis mosaic 
            virus (MMV) full-length transcript promoter in transgenic plants
  JOURNAL   Plant Mol. Biol. 40 (5), 771-782 (1999)
  PUBMED    10487212
REFERENCE   3  (bases 1 to 4453)
  AUTHORS   Maiti IB, Dey N, Shepherd RJ.
  TITLE     Use of the full-length transcript (FLt) from Mirabilis mosaic 
            caulimovirus to express chimeric genes in plants
  JOURNAL   Patent: US US6420547B1-B 16-JUL-2002;
REFERENCE   4  (bases 1 to 4453)
  AUTHORS   Maiti IB, Dey N.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2015) KTRDC, Plant Genetic Engineering Research 
            and Services, College of Agriculture, University of Kentucky, 1401 
            University Drive, Lexington, KY 40546, USA
REFERENCE   5  (bases 1 to 4453)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Transient gene expression vector pUCPMA-M24"
                     /mol_type="other DNA"
                     /db_xref="taxon:1781625"
     regulatory      complement(1..646)
                     /regulatory_class="polyA_signal_sequence"
                     /note="rbcSE9 terminator sequence; derived from pea"
     terminator      6..647
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     misc_feature    complement(647..692)
                     /note="multiple cloning site; XbaI-SacI-XhoI-BamHI-HindIII"
     regulatory      complement(692..1320)
                     /regulatory_class="promoter"
                     /note="M24 promoter; derived from Mirabilis mosaic virus 
                     (MMV)"
     misc_feature    complement(1321..1326)
                     /label=EcoRI restriction enzyme site
                     /note="EcoRI restriction enzyme site"
     misc_feature    1327..4453
                     /label=pUC119
                     /note="pUC119"
     primer_bind     complement(1327..1343)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1556..2011
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     regulatory      2268..2397
                     /regulatory_class="promoter"
                     /note="ampR promoter"
     promoter        2293..2397
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2398..3258
                     /codon_start=1
                     /gene="ampR"
                     /product="beta-lactamase"
                     /label=ampR
                     /note="confers resistance to ampicillin"
                     /protein_id="AMB51376.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            2398..3258
                     /gene="ampR"
                     /label=ampR
     regulatory      3259..3417
                     /regulatory_class="polyA_signal_sequence"
                     /note="ampR terminator"
     rep_origin      3418..4032
                     /label=ColE1 origin of replication
                     /note="ColE1 origin of replication"
     rep_origin      3429..4017
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4305..4326
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4341..4371
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4379..4395
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4403..4419
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"