LOCUS       Exported                4791 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Bacterial expression vector pUChTNFR55, complete sequence.
ACCESSION   LT727637
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4791)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Bacterial expression vector pUChTNFR55"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 2276."
                     /db_xref="taxon:1944712"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    405..581
                     /label=5 UTR of human TNFR55
                     /note="5 UTR of human TNFR55"
     CDS             582..668
                     /codon_start=1
                     /note="unnamed protein product; human TNFR55 signal 
                     sequence"
                     /protein_id="SJL89052.1"
                     /translation="MGLSTVPDLLLPLVLLELLVGIYPSGVIG"
     CDS             669..1949
                     /codon_start=1
                     /note="unnamed protein product; human TNFR55 mature 
                     sequence"
                     /protein_id="SJL89053.1"
                     /translation="MVPHLGDREKRDSVCPQGKYIHPQNNSICCTKCHKGTYLYNDCPG
                     PGQDTDCRECESGSFTASENHLRHCLSCSKCRKEMGQVEISSCTVDRDTVCGCRKNQYR
                     HYWSENLFQCFNCSLCLNGTVHLSCQEKQNTVCTCHAGFFLRENECVSCSNCKKSLECT
                     KLCLPQIENVKGTEDSGTTVLLPLVIFFGLCLLSLLFIGLMYRYQRWKSKLYSIVCGKS
                     TPEKEGELEGTTTKPLAPNPSFSPTPGFTPTLGFSPVPSSTFTSSSTYTPGDCPNFAAP
                     RREVAPPYQGADPILATALASDPIPNPLQKWEDSAHKPQSLDTDDPATLYAVVENVPPL
                     RWKEFVRRLGLSDHEIDRLELQNGRCLREAQYSMLATWRRRTPRREATLELLGRVLRDM
                     DLLGCLEDIEEALCGPAALPPAPSLLR"
     misc_feature    1950..2490
                     /label=3 UTR of human TNFR55
                     /note="3 UTR of human TNFR55"
     misc_feature    2501..2557
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2570..2586)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2594..2610
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      complement(2613..2696)
                     /regulatory_class="promoter"
                     /note="lac promoter"
     promoter        complement(2618..2648)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2663..2684
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(2788..3190)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(2972..3560)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3731..4591)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL89054.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4592..4696)
                     /gene="bla"
                     /label=AmpR promoter