LOCUS       Exported                4279 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Baculovirus expression vector pUCDMIG, complete sequence.
ACCESSION   JN596960
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Yao L, Sun J.
  TITLE     Construction of a baculovirus-silkworm multigene expression system 
            and its application on producing virus-like particles
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Yao L, Sun J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2011) Hen Nan Provincial Key Laboratory of Funiu 
            Mountain Insect Biology, Nan Yang Normal University, 1638 Wolong 
            Road, Nan Yang, He Nan 473061, China
REFERENCE   3  (bases 1 to 4279)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Baculovirus expression vector pUCDMIG"
                     /mol_type="other DNA"
                     /db_xref="taxon:1129737"
     rep_origin      6..362
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     protein_bind    415..448
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      complement(470..751)
                     /regulatory_class="polyA_signal_sequence"
                     /note="HSV tk polyA"
     polyA_signal    638..686
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     regulatory      complement(816..932)
                     /regulatory_class="promoter"
                     /note="p10"
     promoter        complement(822..931)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     regulatory      975..1097
                     /regulatory_class="promoter"
                     /note="polh"
     promoter        976..1067
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     5UTR           1175..1754
                     /note="5-UTR internal ribosome entry site (IRES) sequence 
                     of Rhopalosiphum padi virus"
     CDS             1764..2483
                     /codon_start=1
                     /gene="EGFP"
                     /product="EGFP"
                     /label=EGFP
                     /protein_id="AEY64191.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     gene            1764..2483
                     /gene="EGFP"
                     /label=EGFP
     regulatory      2521..2760
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 polyA"
     polyA_signal    2626..2760
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3131..3233
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             3234..3893
                     /codon_start=1
                     /gene="CmR"
                     /product="chloramphenicol resistance protein"
                     /label=CmR
                     /protein_id="AEY64192.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            3234..3893
                     /gene="CmR"
                     /label=CmR