LOCUS       Exported                3563 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Fluorescent tagging vector pUC57-PS12-turbo-yfp, complete sequence.
ACCESSION   HM150703
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Norris MH, Kang Y, Hoang TT.
  TITLE     Stable site-specific fluorescent tagging tools optimized for 
            burkholderia species
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Norris MH, Kang Y, Hoang TT.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2010) Microbiology, University of Hawaii at Manoa,
            2538 Mccarthy Mall Snyder 310, Honolulu, HI 96822, USA
REFERENCE   3  (bases 1 to 3563)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Fluorescent tagging vector
                     pUC57-PS12-turbo-yfp"
                     /mol_type="other DNA"
                     /db_xref="taxon:862779"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             523..1254
                     /codon_start=1
                     /gene="yfp"
                     /product="turbo yfp"
                     /label=yfp
                     /note="rapidly maturing yellow fluorescent protein"
                     /protein_id="ADK12949.1"
                     /translation="MSSGALLFHGKIPYVVEMEGNVDGHTFSIRGKGYGDASVGKVDAQ
                     FICTTGDVPVPWSTLVTTLTYGAQCFAKYGPELKDFYKSCMPDGYVQERTITFEGDGNF
                     KTRAEVTFENGSVYNRVKLNGQGFKKDGHVLGKNLEFNFTPHCLYIWGDQANHGLKSAF
                     KICHEITGSKGDFIVADHTQMNTPIGGGPVHVPEYHHMSYHVKLSKDVTDHRDNMSLKE
                     TVRAVDCRKTYDFDAGSGDTS"
     gene            523..1254
                     /gene="yfp"
                     /label=yfp
     CDS             523..1221
                     /codon_start=1
                     /product="Phialidium yellow fluorescent proteim"
                     /label=PhiYFP
                     /note="mammalian codon-optimized"
                     /translation="MSSGALLFHGKIPYVVEMEGNVDGHTFSIRGKGYGDASVGKVDAQ
                     FICTTGDVPVPWSTLVTTLTYGAQCFAKYGPELKDFYKSCMPDGYVQERTITFEGDGNF
                     KTRAEVTFENGSVYNRVKLNGQGFKKDGHVLGKNLEFNFTPHCLYIWGDQANHGLKSAF
                     KICHEITGSKGDFIVADHTQMNTPIGGGPVHVPEYHHMSYHVKLSKDVTDHRDNMSLKE
                     TVRAVDCRKTY"
     primer_bind     complement(1342..1358)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1366..1382
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1390..1420)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1435..1456
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1744..2332)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2503..3363)
                     /codon_start=1
                     /gene="bla"
                     /product="beta lactamase"
                     /label=bla
                     /protein_id="ADK12950.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(2503..3363)
                     /gene="bla"
                     /label=bla
     promoter        complement(3364..3468)
                     /gene="bla"
                     /label=AmpR promoter