LOCUS       Exported                3532 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUC19TpTer, complete sequence.
ACCESSION   KX527624
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Shastri S, Spiewak HL, Sofoluwe A, Eidsvaag VA, Asghar AH, Pereira 
            T, Bull EH, Butt AT, Thomas MS.
  TITLE     An efficient system for the generation of marked genetic mutants in 
            members of the genus Burkholderia
  JOURNAL   Plasmid (2016) In press
  PUBMED    27825973
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Shastri S, Spiewak HL, Sofoluwe A, Eidsvaag VA, Asghar AH, Pereira 
            T, Bull EH, Thomas MS.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2016) Department of Infection, Immunity & 
            Cardiovascular Disease, The University of Sheffield, The Medical 
            School, Beech Hill Road, Sheffield, South Yorkshire S10 2RX, UK
REFERENCE   3  (bases 1 to 3532)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Cloning vector pUC19TpTer"
                     /mol_type="other DNA"
                     /db_xref="taxon:1918677"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      425..453
                     /regulatory_class="promoter"
                     /note="Pc promoter; class 1 integron promoter"
     promoter        425..453
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             779..1015
                     /codon_start=1
                     /gene="dfrB2"
                     /product="dihydrofolate reductase"
                     /label=dfrB2
                     /note="confers resistance to trimethoprim"
                     /protein_id="APD15330.1"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     gene            779..1015
                     /gene="dfrB2"
                     /label=dfrB2
     terminator      1039..1125
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     regulatory      1042..1085
                     /regulatory_class="terminator"
                     /note="rrnB T1 terminator; transcription terminator T1 from
                     the E. coli rrnB gene"
     regulatory      1217..1244
                     /regulatory_class="terminator"
                     /note="rrnB T2 terminator; transcription terminator T2 from
                     the E. coli rrnB gene"
     terminator      1217..1244
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1311..1327)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1335..1351
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
     regulatory      complement(1359..1389)
                     /regulatory_class="promoter"
                     /note="lac promoter; promoter for the E. coli lac operon"
     promoter        complement(1359..1389)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1404..1425
                     /label=E. coli catabolite activator protein binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
     rep_origin      complement(1713..2301)
                     /direction=LEFT
                     /note="oriR; high-copy-number ColE1/pMB1/pBR322/pUC origin 
                     of replication"
     CDS             complement(2472..3332)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /protein_id="APD15331.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(2472..3332)
                     /gene="bla"
                     /label=bla
     regulatory      complement(3333..3437)
                     /regulatory_class="promoter"
                     /note="bla promoter"
     promoter        complement(3333..3437)
                     /gene="bla"
                     /label=AmpR promoter