LOCUS       Exported               12082 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUC18_LexA+Cp190, complete sequence.
ACCESSION   KX357893
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12082)
  AUTHORS   Posukh OV, Maksimov DA, Koryakov DE, Pokholkova GV, Skvortsova KN, 
            Belyaeva ES, Belyakin SN.
  TITLE     Repressive Histone Marks Renewal Impedes Replication Fork 
            Progression and Contributes To Late Replication in Drosophila
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12082)
  AUTHORS   Posukh OV, Maksimov DA, Koryakov DE, Pokholkova GV, Skvortsova KN, 
            Belyaeva ES, Belyakin SN.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUN-2016) Genomics lab, IMCB SD RAS, Lavrentjeva ave. 
            8/2, Novosibirsk, NSO 630090, Russia
REFERENCE   3  (bases 1 to 12082)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12082
                     /organism="Cloning vector pUC18_LexA+Cp190"
                     /mol_type="other DNA"
                     /db_xref="taxon:1943578"
     protein_bind    220..289
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    534..567
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      622..2002
                     /regulatory_class="promoter"
     promoter        1448..1900
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     complement(1970..1986)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2004..5942
                     /codon_start=1
                     /product="LexA+CP190"
                     /label=LexA+CP190
                     /note="LexA with CP190 cDNA"
                     /protein_id="AQA27273.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWLELAPKKKRKVEFPRMGEVKS
                     VKVDNWGVFFLQKLQNFFNKTDYCDLTLQFRDNSQLKVHRLVLSACTDYFNVLEQTCEI
                     VDDALIMPNEFQADVVVPIVNFMYTGTLEFELKMYGKLLRTAKEMNMTVLLKLLEAHRR
                     TMENVNRQQRPPSPKGIRRRTVGQPSSGLPQQRVLGPSPQSRNVATPIAQRANTQRGST
                     GNTMSRTSGGSNRSPYGDSSNVKQEPTSPFEQLRKGYNNNKRPAQTSLLSPPSKKPSLE
                     EVKEFAEQQRMRKQIAAEYGDNDPEYDGGMLYDDVHAGDDDDDDMPPQPSTSKQQSPQG
                     TQTQLEHGSTTIILKQDSPSQTPTIIVKDSSNAKLNHTKIIAEVLRQYPHIVKGHKNIK
                     LKIMPNTPAAPTEKSAPATVKPPANQSSATTSPHKKLHVSFKADKSTPLITAQQKAASS
                     QQKSGTSQTTGNQGTGANPPANTAAAQKRRIDSKTMHALIAQGAENTTGPWLCLRCGVN
                     GRPISIPSYRGFRRHLINTHKETIDPALCEHCGWRSVNNRELHFHMYMEHQTKSLLYTF
                     AECALCNQSYRTKGELEAHINEVHTDDNKQQCIYCNKVFEQELQLYRHMKSYHKEQALE
                     DGIIDETDEEFLGSQDEEEEAEGDEEQEPEQTGKVRILSDISLPATSAITVQQAQQEQL
                     QEEDVEQVQQEVKFVGADGNEVELTDEQRKEILSQLNQQQAGATAGGVVMVLSEPEAEH
                     VKQETDEKSLAGTEEEYDDSQIYSELGAADSVESAKKNIADESKESIDNLEWAENLIAE
                     SEEQSNKEPKSDKPRDDISEKLKELTGDWTEDENDDDVDDKPATAELASELANKDPEPT
                     VHEEEDDIDLALQSLHKGPEEATEEKASEESVTSADDAVDAVPNINSQPEKMDVDSEAA
                     DEKASKAEVQIKKEAELENDQEEFIKEDSPIPHSDSVAELREAVTASEGEDDVHLEADN
                     IRKELLDELIAEAEKPDQEKDIVQSEENATTEALDRSVTDEDDLVPPTQVSTEQMEIDE
                     PAAEKAAENNEDTRTADEKEAVEDKPNQTQDVTTAEKPTLESAKAGDEATSGEAASVDK
                     VKSLISEWGDDDEDEDENGVSAAAKEEL"
     CDS             2004..2609
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5-CTGTATATATATACAG-3."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     CDS             2619..2639
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    10161..10194
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      complement(10263..10851)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11022..11882)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11883..11987)
                     /gene="bla"
                     /label=AmpR promoter