LOCUS       Exported                4289 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUC18T-mini-Tn7T-Zeo, complete sequence.
ACCESSION   DQ493876
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Choi K-H., Schweizer HP.
  TITLE     Mini-Tn7 insertion in bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Choi K-H., Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2006) Microbiology, Immunology and Pathology, 
            Colorado State University, 1682 Campus Delivery, Fort Collins, CO 
            80523-1682, USA
REFERENCE   3  (bases 1 to 4289)
  AUTHORS   Choi K-H., Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAY-2010) Microbiology, Immunology and Pathology, 
            Colorado State University, 1682 Campus delivery, Ft. Collins, CO 
            80523, USA
REFERENCE   4  (bases 1 to 4289)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On May 25, 2010 this sequence version replaced DQ493876.1.
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Cloning vector pUC18T-mini-Tn7T-Zeo"
                     /mol_type="other DNA"
                     /db_xref="taxon:383996"
     mobile_element  382..1971
                     /mobile_element_type="transposon:mini-Tn7"
     mobile_element  382..580
                     /mobile_element_type="transposon:Tn7R"
                     /note="right end of Tn7"
     primer_bind     585..601
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      629..723
                     /regulatory_class="terminator"
                     /note="bacteriophage lambda T0"
     terminator      629..723
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     regulatory      820..917
                     /regulatory_class="terminator"
                     /note="Escherichia coli rrnB T1"
     terminator      826..912
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     misc_feature    958..1005
                     /note="FRT; Flp recombinase target site"
     protein_bind    958..1005
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(1116..1490)
                     /codon_start=1
                     /gene="ble"
                     /product="zeocin resistance protein"
                     /label=ble
                     /protein_id="ABF74525.1"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     gene            complement(1116..1490)
                     /gene="ble"
                     /label=ble
     regulatory      complement(1491..1557)
                     /regulatory_class="promoter"
                     /note="pEM7"
     promoter        complement(1509..1556)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     misc_feature    1646..1693
                     /note="FRT; Flp recombinase target site"
     protein_bind    1646..1693
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    1720..1797
                     /note="MCS; multiple cloning site"
     mobile_element  1806..1971
                     /mobile_element_type="transposon:Tn7L"
                     /note="left end of Tn7"
     misc_feature    2123..2391
                     /note="oriT; origin of transfer"
     oriT            2130..2238
                     /note="incP origin of transfer"
     rep_origin      complement(2470..3058)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3229..4089)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="ABF74526.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3229..4089)
                     /gene="bla"
                     /label=bla
     promoter        complement(4090..4194)
                     /gene="bla"
                     /label=AmpR promoter