LOCUS       Exported                5646 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUC-GM-INT, complete sequence.
ACCESSION   AF025392
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Schweizer HD.
  TITLE     Small broad-host-range gentamycin resistance gene cassettes for 
            site-specific insertion and deletion mutagenesis
  JOURNAL   BioTechniques 15 (5), 831-834 (1993)
  PUBMED    8267974
REFERENCE   2  (bases 1 to 5646)
  AUTHORS   Mahenthiralingam E, Marklund BI, Brooks LA, Smith DA, Bancroft GJ, 
            Stokes RW.
  TITLE     Site-directed mutagenesis of the 19-kilodalton lipoprotein antigen 
            reveals No essential role for the protein in the growth and 
            virulence of Mycobacterium intracellulare
  JOURNAL   Infect. Immun. 66 (8), 3626-3634 (1998)
  PUBMED    9673242
REFERENCE   3  (bases 1 to 5646)
  AUTHORS   Mahenthiralingam E, Marklund B-I., Brooks LA, Smith DA, Bancroft GJ,
            Stokes RW.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-1997) Pediatrics, University of British Columbia, 
            950 West 28th Avenue, Vancouver, BC V5Z 4H4, Canada
REFERENCE   4  (bases 1 to 5646)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Cloning vector pUC-GM-INT"
                     /mol_type="genomic DNA"
                     /note="L5-integrase based positive selection mycobacterial 
                     cloning cassette"
                     /db_xref="taxon:67802"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        453..481
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             670..1203
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyl transferase"
                     /function="gentamycin-resistance"
                     /label=aacC1
                     /protein_id="AAC31620.1"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     gene            670..1203
                     /gene="aacC1"
                     /label=aacC1
     misc_feature    1662..1740
                     /note="encodes mycobacteriophage attachment site, attP 
                     core"
     CDS             1989..2987
                     /codon_start=1
                     /product="L5-integrase"
                     /label=L5-integrase
                     /note="mycobacteriophage integrase"
                     /protein_id="AAC31622.1"
                     /translation="MDAEAWLAGEKRLIEMETWTPPQDRAKKAAASAITLEEYTRKWLV
                     ERDLADGTRDLYSGHAERRIYPVLGEVAVTEMTPALVRAWWAGMGRKHPTARRHAYNVL
                     RAVMNTAVEDKLIAENPCRIEQKAADERDVEALTPEELDIVAAEIFEHYRIAAYILAWT
                     SLRFGELIELRRKDIVDDGMTMKLRVRRGASRVGNKIVVGNAKTVRSKRPVTVPPHVAE
                     MIRAHMKDRTKMNKGPEAFLVTTTQGNRLSKSAFTKSLKRGYAKIGRPELRIHDLRAVG
                     ATFAAQAGATTKELMARLGHTTPRMAMKYQMASEARDEAIAEAMSKLAKTS"
     primer_bind     complement(3425..3441)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3449..3465
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3473..3503)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3518..3539
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3827..4415)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4586..5446)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="AAC31621.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(4586..5446)
                     /gene="bla"
                     /label=bla
     promoter        complement(5447..5551)
                     /gene="bla"
                     /label=AmpR promoter