LOCUS       Exported                5522 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pUbiSXR, complete sequence.
ACCESSION   AY452753
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Vickers CE, Xue GP, Gresshoff PM.
  TITLE     A synthetic xylanase as a novel reporter in plants
  JOURNAL   Plant Cell Rep. 22 (2), 135-140 (2003)
  PUBMED    12845475
REFERENCE   2  (bases 1 to 5522)
  AUTHORS   Vickers CE, Xue GP.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2003) Plant Industry, CSIRO, Level 4, Queensland 
            Bioscience Precinct, 306 Carmody Rd, St. Lucia, Brisbane, QLD 4067, 
            Australia
REFERENCE   3  (bases 1 to 5522)
  AUTHORS   Vickers CE, Gresshoff PM.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-DEC-2003) ARC Center for Integrative Legume Research, 
            The University of Queensland, John Hines Building (69), St. Lucia, 
            Brisbane, QLD 4072, Australia
REFERENCE   4  (bases 1 to 5522)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Reporter vector pUbiSXR"
                     /mol_type="other DNA"
                     /db_xref="taxon:255328"
     regulatory      complement(join(1..17,5520..5522))
                     /regulatory_class="promoter"
                     /note="SP6 RNA polymerase promoter"
     misc_feature    267..2247
                     /label=derived from cloning vector pBR322
                     /note="derived from cloning vector pBR322"
     promoter        363..467
                     /gene="bla"
                     /label=AmpR promoter
     CDS             468..1328
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="ampicillin resistance protein"
                     /protein_id="AAR29600.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            468..1328
                     /gene="bla"
                     /label=bla
     rep_origin      1499..2087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      complement(2345..2367)
                     /regulatory_class="promoter"
                     /note="T7 RNA polymerase promoter"
     promoter        2345..2363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(2362)
                     /label=T7 RNA polymerase transcription initiation site
                     /note="T7 RNA polymerase transcription initiation site"
     promoter        2393..4384
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     regulatory      2398..3374
                     /regulatory_class="promoter"
                     /note="maize ubiquitin Ubi-1 promoter"
     intron          3375..4384
                     /note="maize ubiquitin Ubi-1 intron 1"
     5UTR           4398..4426
                     /label=synthetic untranslated leader sequence
                     /note="synthetic untranslated leader sequence"
     CDS             4427..5140
                     /codon_start=1
                     /gene="sXynA"
                     /product="xylanase"
                     /label=sXynA
                     /note="synthetic xylanase; reporter gene"
                     /protein_id="AAR29601.1"
                     /translation="MASNGKKFTVGNGQNQHKGVNDGFSYEIWLDNTGGNGSMTLGSGA
                     TFKAEWNAAVNRGNFLARRGLDFGSQKKATDYDYIGLDYAATYKQTASASGNSRLCVYG
                     WFQNRGLNGVPLVEYYIIEDWVDWVPDAQGKMVTIDGAQYKIFQMDHTGPTINGGSETF
                     KQYFSVRQQKRTSGHITVSDHFKEWAKQGWGIGNLYEVALNAEGWQSSGVADVTLLDVY
                     TTPKGSSPATSAAPR"
     gene            4427..5140
                     /gene="sXynA"
                     /label=sXynA
     regulatory      5162..5499
                     /regulatory_class="terminator"
                     /note="rbcS 3 terminator"
     promoter        complement(5521..17)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    complement(5522)
                     /label=SP6 RNA polymerase transcription initiation site
                     /note="SP6 RNA polymerase transcription initiation site"