LOCUS       Exported                7018 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pUbiGUSPlus, complete sequence.
ACCESSION   AY452736
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7018)
  AUTHORS   Vickers CE, Xue GP, Gresshoff PM.
  TITLE     A synthetic xylanase as a novel reporter in plants
  JOURNAL   Plant Cell Rep. 22 (2), 135-140 (2003)
  PUBMED    12845475
REFERENCE   2  (bases 1 to 7018)
  AUTHORS   Vickers CE.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2003) ARC Centre for Integrative Legume Research, 
            The University of Queensland, Room 213, John Hines Building (69), 
            St. Lucia, QLD 4072, Australia
REFERENCE   3  (bases 1 to 7018)
  AUTHORS   Vickers CE.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-FEB-2004) ARC Centre for Integrative Legume Research, 
            The University of Queensland, Room 213, John Hines Building (69), 
            St. Lucia, QLD 4072, Australia
REFERENCE   4  (bases 1 to 7018)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On Feb 20, 2004 this sequence version replaced AY452736.1.
FEATURES             Location/Qualifiers
     source          1..7018
                     /organism="Reporter vector pUbiGUSPlus"
                     /mol_type="other DNA"
                     /db_xref="taxon:257025"
     primer_bind     319..335
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        351..2342
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     regulatory      356..1331
                     /regulatory_class="promoter"
                     /note="from Maize ubiquitin gene; Ubi-1"
     intron          1332..2341
                     /note="intron 1 from maize ubiquitin gene; Ubi-1"
     misc_feature    2356..2388
                     /note="synthetic untranslated leader sequence; sUTR"
     CDS             join(2389..2403,2594..4441)
                     /codon_start=1
                     /product="GUSPlus-His6"
                     /label=GUSPlus-His6
                     /note="beta-glucuronidase"
                     /protein_id="AAR29087.1"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKNASHHHHHHV"
     CDS             join(2389..2403,2594..4411)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          2404..2593
                     /note="catalase intron"
     CDS             4418..4435
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      4467..4719
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      4470..4722
                     /regulatory_class="terminator"
                     /note="from nopaline synthase gene; NOS"
     regulatory      4486..4491
                     /regulatory_class="polyA_signal_sequence"
                     /note="from nopaline synthase gene; NOS"
     primer_bind     complement(4737..4753)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4761..4777
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4785..4815)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4830..4851
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    4952..6959
                     /label=sequence from cloning vector pBR322
                     /note="sequence from cloning vector pBR322"
     rep_origin      complement(5139..5727)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5898..6758)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="ampicillin resistance protein; bla"
                     /protein_id="AAR29088.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6759..6863)
                     /gene="bla"
                     /label=AmpR promoter