LOCUS       Exported                9420 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUAST-R57 DNA, complete sequence.
ACCESSION   AB233207
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9420)
  AUTHORS   Takahashi K, Ueda R.
  TITLE     Cloning vector pUAST-R57
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9420)
  AUTHORS   Ueda R, Takahashi K.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2005) Kuniaki Takahashi, National Institute of 
            Genetics, Genetic Strains Research Center; 1,111 Yata, Mishima, 
            Shizuoka 411-8540, Japan (E-mail:kuntakah@lab.nig.ac.jp, 
            Tel:81-55-981-6824, Fax:81-55-981-6825)
REFERENCE   3  (bases 1 to 9420)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9420
                     /organism="Cloning vector pUAST-R57"
                     /mol_type="other DNA"
                     /db_xref="taxon:343456"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(994..1854)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    2761..2993
                     /label=P element 3 end
                     /note="P element 3 end"
     protein_bind    3038..3132
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3151..3389
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     misc_feature    3389..3474
                     /note="polylinker; unique cloning sites for BglII, NotI 
                     CpoI, KpnI"
     CDS             join(3475..3482,3538..3676,3737..3756)
                     /codon_start=1
                     /gene="Dret"
                     /product="Ret oncogene"
                     /label=Dret
                     /note="Drosophila ret partial sequence"
                     /protein_id="BAE44178.1"
                     /translation="ELKLVHRDLAARNVLLADGKICKISDFGLTRDVYEDDAYLKRSRD
                     RVPVKWMAPE"
     gene            join(3475..3482,3538..3676,3737..3756)
                     /gene="Dret"
                     /label=Dret
     misc_feature    3757..3818
                     /note="polylinker; unique cloning sites for SfiI, XbaI"
     intron          3896..3961
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4091..4111
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4536..4670
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             join(5175..5246,5641..5914,5989..6643,6705..7020,7241..7372,
                     7443..8057)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    8835..9420
                     /label=P element 5 end