LOCUS       Exported               14213 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUAST-attB_X6S1-HA, complete sequence.
ACCESSION   KT921800
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14213)
  AUTHORS   Posukh OV, Maksimov DA, Koryakov DE, Pokholkova GV, Skvortsova KN, 
            Belyaeva ES, Belyakin SN.
  TITLE     Repressive histone marks renewal impedes replication fork 
            progression and contributes to late replication in Drosophila
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14213)
  AUTHORS   Belyakin SN.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2015) Genomics Lab, IMCB SD RAS, Lavrentjeva Ave. 
            8/2, Novosibirsk, NSO 630090, Russia
REFERENCE   3  (bases 1 to 14213)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..14213
                     /organism="Cloning vector pUAST-attB_X6S1-HA"
                     /mol_type="other DNA"
                     /db_xref="taxon:1816701"
     protein_bind    48..142
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     regulatory      159..386
                     /regulatory_class="promoter"
                     /note="SuUR"
     promoter        161..399
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     5UTR           507..904
     CDS             join(905..1232,1340..1539,1606..1784,1871..4094)
                     /codon_start=1
                     /product="SuUR-HA"
                     /label=SuUR-HA
                     /protein_id="AMR70306.1"
                     /translation="MYHFVSEQTPEVRLTDEALVTSHVTQYLKSFQLDAVRFVYDRLAK
                     REFCILNDESGLGKVATVAALLSALPPAKKTLVVLQNDEQLLTGWRFHLDTLTDLQVYI
                     IQGVQDTTDSPHSVYLAKWSQLRSIGDLSRLKFDYIMVDNRGHSLNNSFCTSMLLKQFE
                     GRVNVLISSVDVTSDVRLLYNVLRLGGRLEHQYKSFASFDRKFHLPDPKEVFSKRIDLE
                     EYYKQRGFLSEYIKDFRLRRFRHQFDKSLPLVAPEQYKHNLNLWLASKNSQSTISGSDV
                     CSTIASIDNNPAQQNKTGLFEETDRLSEHSVDDVAMSPLIFEYSESDDEPLTVEPDADQ
                     NPVLVVSSDDCEIVTPPSTPQNRTPSLNESPRTKSKKKFSKKTSPRKKADLTDSEEDDE
                     ATDNMPPRKRTRAATVHLTPKTRRLNVRILRVSLDTLSTPPPSRTTTAIVTPKTEPTAR
                     RKNLKKRTVSPVDVGRPATRGMQRLTRSAETKINSKYLKHRSLDDVKRSFPRRVKLEGN
                     QTPRSSKQIVKQEPKSKVGQEKKQKTVDVPAQGTAKRKPGRPRKCQTKTEDLGKTKTKP
                     NSKHLPPTPQVLSGSSLSSEYMQCAQRIPDNLDAIESPAFRVPFTPQQTPMLLTLPSTH
                     NLLNDSEVVSIPLYKDPVETVVINSSHDECSPQDPSQSRRTKALKRKRKPVTSVNSSFG
                     GGLGLPPAKRSANKSPDLFSISSEHSQIPLAQPRPSSPFEGFKIFGSEVKQFQQQLAKV
                     NISVPKKKRDRSCLDILEQMFEPRQQQSAKTSPKVLPTLPLTQKDDAESTITQRRRTLL
                     EDDFFEITNNGQFGSRMRLNSSGEVSPVQPDQQSVRPSQANKITNYLIGSGITQERTQP
                     SNGNRNSILASLRKSPKSPKQGAKSTQATKLTRWFGSVFGGGASQTSSVESVSAPSTPV
                     NSSTSAAACQTRSARSGGASGPTKRKRLELFKSMYPYDVPDYAAMG"
     CDS             4056..4082
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     3UTR           4095..4574
     intron          5515..5580
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5710..5730
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6002..6136
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    10212..10281
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    10635..11220
                     /label=P element 5 end
     rep_origin      complement(11455..12043)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12214..13074)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(13075..13179)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    13981..14213
                     /label=P element 3 end
                     /note="P element 3 end"