LOCUS       Exported               14300 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUAST-attB-stop-SuVar-MycDam, complete sequence.
ACCESSION   KT921803
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14300)
  AUTHORS   Posukh OV, Maksimov DA, Koryakov DE, Pokholkova GV, Skvortsova KN, 
            Belyaeva ES, Belyakin SN.
  TITLE     Repressive histone marks renewal impedes replication fork 
            progression and contributes to late replication in Drosophila
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14300)
  AUTHORS   Belyakin SN.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2015) Genomics Lab, IMCB SD RAS, Lavrentjeva Ave. 
            8/2, Novosibirsk, NSO 630090, Russia
REFERENCE   3  (bases 1 to 14300)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..14300
                     /organism="Cloning vector pUAST-attB-stop-SuVar-MycDam"
                     /mol_type="other DNA"
                     /db_xref="taxon:1816700"
     protein_bind    62..156
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     regulatory      173..400
                     /regulatory_class="promoter"
     promoter        175..413
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     protein_bind    469..502
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     polyA_signal    529..1401
                     /label=hsp70 poly(A)
                     /note="polyadenlyation signal from the Drosophila 
                     melanogaster hsp70Ab gene"
     protein_bind    2585..2618
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             2718..5510
                     /codon_start=1
                     /product="Su(var)3-9-Myc-Dam"
                     /label=Su
                     /protein_id="AMR68904.1"
                     /translation="MATAEAQIGVNRNLQKQDLSNLDVSKLTPLSPEVISRQATINIGT
                     IGHVAHGKSTVVKAISGVQTVRFKNELERNITIKLERLSEKKIKNLLTSKQQRQQYEIK
                     QRSMLRHLAELRRHSRFRRLCTKPASSSMPASTSSVDRRTTRRSTSQTSLSPSNSSGYG
                     SVFGCEEHDVDKIPSLNGFAKLKRRRSSCVGAPTPNSKRSKNNMGVIAKRPPKGEYVVE
                     RIECVEMDQYQPVFFVKWLGYHDSENTWESLANVADCAEMEKFVERHQQLYETYIAKIT
                     TELEKQLEALPLMENITVAEVDAYEPLNLQIDLILLAQYRAAGSRSQREPQKIGERALK
                     SMQIKRAQFVRRKQLADLALFEKRMNHVEKPSPPIRVENNIDLDTIDSNFMYIHDNIIG
                     KDVPKPEAGIVGCKCTEDTEECTASTKCCARFAGELFAYERSTRRLRLRPGSAIYECNS
                     RCSCDSSCSNRLVQHGRQVPLVLFKTANGSGWGVQAATALRKGEFVCEYIGEIITSDEA
                     NERGKAYDDNGRTYLFDLDYNTAQDSEYTIDAANYGNISHFINHSCDPNLAVFPCWIEH
                     LNVALPHLVFFTLRPIKAGEELSFDYIRADNEDVPYENLSTAVRVECRCGADNCRKVLF
                     AAAAGEQKLISEEDLAPGKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVF
                     LNTDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKS
                     QDPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVER
                     HIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPAKK
                     "
     CDS             4638..4667
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          5602..5667
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5797..5817
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6089..6223
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    10299..10368
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    10722..11307
                     /label=P element 5 end
     rep_origin      complement(11542..12130)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12301..13161)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(13162..13266)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    14068..14300
                     /label=P element 3 end
                     /note="P element 3 end"