LOCUS       Exported               14180 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUAST-attB-hsp70-stop-dam-comr, complete sequence.
ACCESSION   KC845569
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14180)
  AUTHORS   Laktionov P, Maksimov D, White-Cooper H, Belyakin S.
  TITLE     Cookie monster binding to the repressed chromosomal domains is 
            needed for selective activation of testis-specific genes in 
            Drosophila melanogaster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14180)
  AUTHORS   Laktionov PP, Belyakin SN.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2013) Genomics lab, Institute of molecular and 
            cellular biology, Lavrentiev ave 8/2, Novosibirsk 630090, Russia
REFERENCE   3  (bases 1 to 14180)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..14180
                     /organism="Cloning vector pUAST-attB-hsp70-stop-dam-comr"
                     /mol_type="other DNA"
                     /db_xref="taxon:1366866"
     protein_bind    48..142
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     regulatory      159..386
                     /regulatory_class="promoter"
                     /note="hsp70 minimal promoter"
     promoter        161..399
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     misc_recomb     455..488
                     /label=loxP recombination site
                     /note="loxP recombination site"
     protein_bind    455..488
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      507..2539
                     /regulatory_class="terminator"
                     /note="hsp70Ab transcription terminator"
     polyA_signal    515..1387
                     /label=hsp70 poly(A)
                     /note="polyadenlyation signal from the Drosophila 
                     melanogaster hsp70Ab gene"
     misc_recomb     2572..2605
                     /label=loxP recombination site
                     /note="loxP recombination site"
     protein_bind    2572..2605
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             2657..5353
                     /codon_start=1
                     /product="DamComr"
                     /label=DamComr
                     /note="DamComr fusion"
                     /protein_id="AGR55452.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLNT
                     DFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQDP
                     FRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFFYCE
                     SYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVERHIP
                     VLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPAKKAGE
                     QKLISEEDLAGADLRPLMSGNQDTLGQSQQVNESGDEYEEDAATSWTNELEQQESRTPA
                     DSPEFLDFVDSEDPLEDFERNPLQKEPLDLAVKPVSDINKKAAENHRRIYRQLGIHIPE
                     TPKSHPLLHYAFVVKLFLATDNSSIHFLKWSANDRELEVDYQGMQEHLSSGCSMFHCRN
                     TLQFTGTLLAHGFERVSLKDVRKSSVTNITLIYRNPNFVKGQLLKLHKLAQECEKIPEI
                     SSPETAEPRRPPLHLPNHAHISRMVRRGDLCSTPYTSRSALQVARCHFQTLLGYQADLG
                     VLKDRNNHDMFNKNIMTQLNVKRGRSSINNAPVDLAPSKVSFEKFVKPHESVINIGIGQ
                     APDYAGYYGQVELFKVNEFFSEYLPRYGSKITGYKEIVMDATNKSIGFQQNLPIGMNYS
                     DDEDDALPPISSDLDLDFMPSTSAAAKMKSTGRKVVEDSDLEQAMQELCGGSNLNEEEE
                     LPQTSTRFKAKPKPRAKTKAKPTKRQAKPVHSSSSECEDFKPDEVYEGFKSRNPKNVKL
                     KPSDENISYTLEEGTDFEEQVIEETTETKVQDSQYDDLDDDDDDEEEDYFDEDDEDYVY
                     KNLQYRNPPEPPKSRRYDLRNSKRNPR"
     CDS             3497..3526
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          5482..5547
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5677..5697
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5969..6103
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     gene            6455..10000
                     /gene="mini-white"
                     /label=mini-white
     misc_recomb     10153..10476
                     /label=phiC31 attB site
                     /note="phiC31 attB site"
     protein_bind    10179..10248
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    10602..11187
                     /label=P element 5 end
     rep_origin      complement(11422..12010)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12181..13041)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="ampicillin resistance"
                     /protein_id="AGR55453.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(12181..13041)
                     /gene="bla"
                     /label=bla
     promoter        complement(13042..13146)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    13948..14180
                     /label=P element 3 end
                     /note="P element 3 end"