LOCUS       Exported               12343 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUAST-attB-Dam plasmid pUAST, complete sequence.
ACCESSION   JN993988
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12343)
  AUTHORS   Belyakin S, Maksimov D.
  TITLE     SUUR binding to chromosomes is developmentally regulated and affects
            tissue-specific genes in Drosophila melanogaster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12343)
  AUTHORS   Belyakin S, Maksimov D.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2011) MCB, ICBFM, Lavrentjeva Ave. 8, Novosibirsk,
            NSO 630090, Russia
REFERENCE   3  (bases 1 to 12343)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12343
                     /organism="Cloning vector pUAST-attB-Dam"
                     /plasmid="pUAST"
                     /mol_type="other DNA"
                     /db_xref="taxon:1157497"
     protein_bind    48..142
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        161..399
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     protein_bind    455..488
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     polyA_signal    515..1387
                     /label=hsp70 poly(A)
                     /note="polyadenlyation signal from the Drosophila 
                     melanogaster hsp70Ab gene"
     protein_bind    2572..2605
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             2657..3490
                     /codon_start=1
                     /gene="Dam"
                     /product="Dam methyltransferase subunit"
                     /label=Dam
                     /protein_id="AFD02332.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLNT
                     DFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQDP
                     FRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFFYCE
                     SYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVERHIP
                     VLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPAKK"
     gene            2657..3490
                     /gene="Dam"
                     /label=Dam
     CDS             3497..3526
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          3645..3710
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3840..3860
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4132..4266
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    8342..8411
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    8765..9350
                     /label=P element 5 end
     rep_origin      complement(9585..10173)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10344..11204)
                     /codon_start=1
                     /gene="Amp-r"
                     /product="ampicillin resistance"
                     /label=Amp-r
                     /protein_id="AFD02331.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(10344..11204)
                     /gene="Amp-r"
                     /label=Amp-r
     promoter        complement(11205..11309)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    12111..12343
                     /label=P element 3 end
                     /note="P element 3 end"