LOCUS       Exported               10052 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUASpDir, complete sequence.
ACCESSION   EU178815
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10052)
  AUTHORS   Jenny A, Mlodzik M.
  TITLE     Modified vectors for the two-step directional cloning of inverted 
            repeats for RNA interference in Drosophila
  JOURNAL   BioTechniques 44 (3), 335-339 (2008)
  PUBMED    18361787
REFERENCE   2  (bases 1 to 10052)
  AUTHORS   Jenny A, Mlodzik M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2007) Brookdale Department of Molecular, Cell and 
            Developmental Biology, Mount Sinai School of Medicine, 1 Gustave L. 
            Levi Place Annenberg 18-92 Box 1020, New York, NY 10029, USA
REFERENCE   3  (bases 1 to 10052)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10052
                     /organism="Cloning vector pUASpDir"
                     /mol_type="other DNA"
                     /note="pUASp-derived RNAi vector for Drosophila 
                     melanogaster"
                     /db_xref="taxon:490160"
     misc_feature    1750..1982
                     /label=P element 3 end
                     /note="P element 3 end"
     promoter        2784..2888
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2889..3749
                     /codon_start=1
                     /gene="bla"
                     /product="AmpR"
                     /label=bla
                     /note="beta lactamase"
                     /protein_id="ABY49798.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            2889..3749
                     /gene="bla"
                     /label=bla
     regulatory      3043..3164
                     /regulatory_class="enhancer"
                     /gene="bla"
                     /note="14 UAS Gal4 sites"
     intron          3468..3541
                     /gene="bla"
                     /note="white intron in hairpin"
     rep_origin      3920..4508
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4743..5328
                     /label=P element 5 end