LOCUS       Exported                6898 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Integrative vector pTYGi9, complete sequence.
ACCESSION   KF957646
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6898)
  AUTHORS   Tianyu Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2013) Center for Infection & Immunity, Guangzhou 
            Institutes of Biomedicine and Health (GIBH), Chinese Academy of 
            Sciences, 190 Kaiyuan Road, Science Park, Guangdong, Guangzhou 
            510530, China
REFERENCE   2  (bases 1 to 6898)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6898
                     /organism="Integrative vector pTYGi9"
                     /mol_type="other DNA"
                     /label=from Mycobacterium tuberculosis H37Rv
                     /note="from Mycobacterium tuberculosis H37Rv"
                     /db_xref="taxon:1929958"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             129..989
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="ampcillin-resistance protein"
                     /protein_id="AHK23747.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            129..989
                     /gene="bla"
                     /label=bla
     rep_origin      1148..1763
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             2804..3919
                     /codon_start=1
                     /gene="int"
                     /product="integrase"
                     /label=int
                     /protein_id="AHK23748.1"
                     /translation="MARRGWGSLKTQRSGRIQASYVNPQDGVRYYALQTYDNKMDAEAW
                     LAGEKRLIEMETWTPPQDRAKKAAASAITLEEYTRKWLVERDLADGTRDLYSGHAERRI
                     YPVLGEVAVTEMTPALVRAWWAGMGRKHPTARRHAYNVLRAVMNTAVEDKLIAENPCRI
                     EQKAADERDVEALTPEELDIVAAEIFEHYRIAAYILAWTSLRFGELIELRRKDIVDDGM
                     TMKLRVRRGASRVGNKIVVGNAKTVRSKRPVTVPPHVAEMIRAHMKDRTKMNKGPEAFL
                     VTTTQGNRLSKSAFTKSLKRGYAKIGRPELRIHDLRAVGATFAAQAGATTKELMARLGH
                     TTPRMAMKYQMASEARDEAIAEAMSKLAKTS"
     gene            2804..3919
                     /gene="int"
                     /label=int
     misc_feature    4378..4405
                     /label=MTB dif
                     /note="MTB dif"
     CDS             4467..5465
                     /codon_start=1
                     /gene="hyg"
                     /product="hygromycin B phosphotransferase"
                     /label=hyg
                     /protein_id="AHK23749.1"
                     /translation="MTQESLLLLDRIDSDDSYASLRNDQEFWEPLARRALEELGLPVPP
                     VLRVPGESTNPVLVGEPGPVIKLFGEHWCGPESLASESEAYAVLADAPVPVPRLLGRGE
                     LRPGTGAWPWPYLVMSRMTGTTWRSAMDGTTDRNALLALARELGRVLGRLHRVPLTGNT
                     VLTPHSEVFPELLRERRAATVEDHRGWGYLSPRLLDRLEDWLPDVDTLLAGREPRFVHG
                     DLHGTNIFVDLAATEVTGIVDFTDVYAGDSRYSLVQLHLNAFRGDREILAALLDGAQWK
                     RTEDFARELLAFTFLHDFEVFEETPLDLSGFTDPEELAQFLWGPPDTAPGA"
     gene            4467..5465
                     /gene="hyg"
                     /label=hyg
     CDS             5521..6240
                     /codon_start=1
                     /gene="eGFP"
                     /product="enhanced green fluorescent protein"
                     /label=eGFP
                     /protein_id="AHK23750.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     gene            5521..6240
                     /gene="eGFP"
                     /label=eGFP
     promoter        complement(6255..6273)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6283..6299)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      6441..6896
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"