LOCUS       Exported                4234 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTW8, complete sequence.
ACCESSION   MG417048
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Hagen KE, Tramp CA, Altermann E, Welker DL, Tompkins TA.
  TITLE     Sequence analysis of plasmid pIR52-1 from Lactobacillus helveticus 
            R0052 and investigation of its origin of replication
  JOURNAL   Plasmid 63 (2), 108-117 (2010)
  PUBMED    20051251
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Hagen KE, Tramp CA, Altermann E, Welker DL, Tompkins TA.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2017) Biology, Utah State University, 5305 Old 
            Main Hill, Logan, UT 84322-5305, USA
REFERENCE   3  (bases 1 to 4234)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Cloning vector pTW8"
                     /mol_type="other DNA"
                     /db_xref="taxon:2315429"
     CDS             complement(108..671)
                     /codon_start=1
                     /gene="repB"
                     /product="DNA replication protein RepB"
                     /label=repB
                     /protein_id="AXU41051.1"
                     /translation="MAKTIKQLADELKVSKQTIQYHYQRLPAKNQQKNSQGTNLISTTA
                     ERIIRSKVAKPLLANKQQIGSKEPTKTSKENNDLIITLRREVEDLKSQRDKQLAAKDQQ
                     ISSKDRQIDHLTKLIDQQQQLQLAIVAENRQLKEHVQKLSGLLEPSSTTQQQQSNDKDD
                     ALSNSEKQKRMHKNKPNKNWWHFW"
     gene            complement(108..671)
                     /gene="repB"
                     /label=repB
     CDS             1265..2371
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /note="plasmid replication initiation protein"
                     /protein_id="AXU41052.1"
                     /translation="MDQTNFKFYQADNVYGALFFQFPKVLMYGEQYRYLSSEAKLAYMV
                     LKDRLEYSLRNHWVDEEDHVYFIFTNQELMDLFNCSKGKLATVKKELEKAGLLYQKAMH
                     FNPKTGKNEPNRLYLAELDMRSTDVYLRGEYGQKEPQTLATSESLKNRPSRETVETLAT
                     SESLKNRPSRKSVDDNPQTLATSESLKNRHDLDKELKERDTNRYNIDTQKLDFSTANFS
                     PAEIETQNRDLVKHANDFLTDEDSGLPVFLEPEAVQLLSFWCRTPQQMRRFIGIILNAK
                     YRVEKDHKDIGVLIPLDDEELKPLMTKALRRYFNALRSNEKHIKNVENYLYGTMQNLFG
                     IWWNKQAAREYAAKHPEEQNTDNEHTWS"
     gene            1265..2371
                     /gene="repA"
                     /label=repA
     CDS             2660..3397
                     /codon_start=1
                     /gene="ermB"
                     /product="erythromycin resistance ErmB"
                     /label=ermB
                     /note="23S (adenine (2058)-N(6)) -methyltransferase"
                     /protein_id="AXU41053.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTT
                     KLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYH
                     LSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFH
                     PKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNN
                     LSTVTYEQVLSIFNSYLLFNGRK"
     gene            2660..3397
                     /gene="ermB"
                     /label=ermB
     regulatory      3580..4168
                     /regulatory_class="replication_regulatory_region"
                     /note="Escherichia coli origin of replication from pUC19"
     rep_origin      3580..4168
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"