LOCUS       Exported               13744 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pTVBMV, complete sequence.
ACCESSION   JQ407081
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13744)
  AUTHORS   Gao R, Tian YP, Wang J, Yin X, Li XD, Valkonen JP.
  TITLE     Construction of an infectious cDNA clone and gene expression vector 
            of Tobacco vein banding mosaic virus (genus Potyvirus)
  JOURNAL   Virus Res. 169 (1), 276-281 (2012)
  PUBMED    22820405
REFERENCE   2  (bases 1 to 13744)
  AUTHORS   Gao R, Tian YP, Wang J, Li XD.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2012) Department of Plant Pathology, Shandong 
            Agricultural University, No. 61, Daizong Street, Taian, Shandong 
            271018, China
REFERENCE   3  (bases 1 to 13744)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13744
                     /organism="Expression vector pTVBMV"
                     /mol_type="other DNA"
                     /db_xref="taxon:1242981"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      418..1251
                     /regulatory_class="promoter"
                     /note="CaMV 35S"
     promoter        910..1255
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    1252..4393
                     /note="derived from Tobacco vein banding mosaic virus 
                     isolate HN39"
     intron          4394..4582
                     /note="intron IV of ST-LS1 gene from Solanum tuberosum"
     misc_feature    4583..5422
                     /note="derived from Tobacco vein banding mosaic virus 
                     isolate HN39"
     intron          5423..5643
                     /note="intron II of NiR gene from Phaseolus vulgaris"
     misc_feature    5644..7042
                     /note="derived from Tobacco vein banding mosaic virus 
                     isolate HN39"
     intron          7043..7231
                     /note="intron IV of ST-LS1 gene from Solanum tuberosum"
     misc_feature    7232..11486
                     /note="derived from Tobacco vein banding mosaic virus 
                     isolate HN39"
     misc_feature    11487..417
                     /label=pUC19 sequence
                     /note="pUC19 sequence"
     primer_bind     complement(11523..11539)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11547..11563
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11571..11601)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11616..11637
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(11925..12513)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12684..13544)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(13545..13649)
                     /gene="bla"
                     /label=AmpR promoter