LOCUS       Exported                4255 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTSL, complete sequence.
ACCESSION   KU314761
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Taylor NM, Prokhorov NS, Guerrero-Ferreira RC, Shneider MM, Browning
            C, Goldie KN, Stahlberg H, Leiman PG.
  TITLE     Structure of the T4 baseplate and its function in triggering sheath 
            contraction
  JOURNAL   Nature 533 (7603), 346-352 (2016)
  PUBMED    27193680
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Shneider MM, Miroshikov KA.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2015) Laboratory of Molecular Bioengineering, 
            IBCH, Miklukho-Maklaya str. 16/10, Moscow 117997, Russian Federation
REFERENCE   3  (bases 1 to 4255)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Cloning vector pTSL"
                     /mol_type="other DNA"
                     /db_xref="taxon:1837086"
     regulatory      complement(26..73)
                     /regulatory_class="terminator"
                     /note="T7"
     terminator      26..73
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(123..815)
                     /codon_start=1
                     /gene="SlyD"
                     /product="SlyD protein"
                     /label=SlyD
                     /note="FKBP-type peptidyl-prolyl cis-trans isomerase SlyD 
                     provided with N-terminal HisTag and C-terminal TEV protease
                     recognition site"
                     /protein_id="ANA08428.1"
                     /translation="MGSSHHHHHHASKVSKDLVVSLAYQVRTEDGVLVDESPVSAPLDY
                     LHGHGSLISGLETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVG
                     MRFLAETDQGPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHV
                     HGAHDHHHDHDHDGQLGGSGGSGGSGGSGGELGENLYFQGSGSEFELRRQACGRTRAPP
                     PPPLRSGC"
     gene            complement(123..815)
                     /gene="SlyD"
                     /label=SlyD
     CDS             complement(140..157)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(207..227)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(786..803)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             828..850
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    865..889
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      complement(889..908)
                     /regulatory_class="promoter"
                     /note="T7"
     promoter        complement(890..908)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1417..1608
                     /codon_start=1
                     /gene="rop"
                     /product="rop"
                     /label=rop
                     /note="plasmid copy number regulation"
                     /protein_id="ANA08429.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     gene            1417..1608
                     /gene="rop"
                     /label=rop
     misc_feature    1710..1852
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      2038..2626
     CDS             complement(2797..3657)
                     /codon_start=1
                     /gene="ampR"
                     /product="ampR"
                     /label=ampR
                     /note="ampicillin resistance protein; confers resistance to
                     ampicillin"
                     /protein_id="ANA08430.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(2797..3657)
                     /gene="ampR"
                     /label=ampR
     regulatory      complement(3658..3762)
                     /regulatory_class="promoter"
                     /note="ampR"
     promoter        complement(3658..3762)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3789..4244)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 ori"