LOCUS       Exported                5483 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Phage display vector pTScDisp, complete sequence.
ACCESSION   AY618909
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Kolesnikov AV, Kozyr AV, Ignatova AN, Zakharova MY.
  TITLE     Improved purification of filamentous bacteriophages for phage 
            display using size-exclusion chromatography
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5483)
  AUTHORS   Kolesnikov AV, Kozyr AV, Ignatova AN, Zakharova MY.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2004) Laboratory of Biocatalysis, Shemyakin and 
            Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 
            16/10, Moscow 117997, Russia
REFERENCE   3  (bases 1 to 5483)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Phage display vector pTScDisp"
                     /mol_type="other DNA"
                     /label=derived from pUC118
                     /note="derived from pUC118"
                     /db_xref="taxon:279975"
     CDS             complement(142..1233)
                     /codon_start=1
                     /gene="lacI"
                     /product="lactose operon repressor"
                     /label=lacI
                     /note="LacI"
                     /protein_id="AAT40742.1"
                     /translation="MVNVKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAM
                     AELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVER
                     SGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGD
                     WSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTED
                     SSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTA
                     SPRALADSLMQLARQVSRLESGQ"
     gene            complement(142..1233)
                     /gene="lacI"
                     /label=lacI
     CDS             complement(142..1224)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(1225..1302)
                     /gene="lacI"
                     /label=lacI promoter
     regulatory      1345..1477
                     /regulatory_class="promoter"
                     /note="Tac"
     protein_bind    1472..1488
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1539..1561
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      1555..1560
                     /regulatory_class="ribosome_binding_site"
                     /note="T7 g10 RBS"
     misc_feature    1572..1631
                     /label=pelB leader for periplasmic migration
                     /note="pelB leader for periplasmic migration"
     misc_feature    1679..1726
                     /label=ScFv linker
                     /note="ScFv linker"
     misc_feature    1787..2326
                     /gene="pIII"
                     /note="m13 minor coat protein fragment; this fragment of 
                     the protein is used for N-terminal fusion expression during
                     phage display"
     gene            1787..2326
                     /gene="pIII"
                     /label=pIII
     regulatory      2363..2791
                     /regulatory_class="terminator"
                     /note="RRNB"
     terminator      2538..2624
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2716..2743
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      2841..3296
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      complement(2925..3221)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 ori"
     promoter        3578..3682
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3683..4543
                     /codon_start=1
                     /gene="Bla"
                     /product="beta-lactamase"
                     /label=Bla
                     /protein_id="AAT40743.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            3683..4543
                     /gene="Bla"
                     /label=Bla
     rep_origin      4714..5302
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      5305..5311
                     /label=ColE1 ori
                     /note="ColE1 ori"