LOCUS       Exported                6334 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pTR_KJUMPS DNA, complete sequence.
ACCESSION   LC276368
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Kasai Y, Matsuzaki K, Ikeda F, Yoshimitsu Y, Harayama S.
  TITLE     Precise excision of a selectable marker gene in transgenic 
            Pseudococcomyxa strains by the piggyBac transposase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6334)
  AUTHORS   Kasai Y, Harayama S.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2017) Contact:Yuki Kasai Chuo University, Research
            and Development Initiative; 1-13-27 Kasuga, Bunkyo-ku, Tokyo 
            112-8551, Japan
REFERENCE   3  (bases 1 to 6334)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6334
                     /organism="Expression vector pTR_KJUMPS"
                     /mol_type="other DNA"
                     /db_xref="taxon:2014527"
     misc_structure  1..2512
                     /label=derived from plasmid pB007
                     /note="derived from plasmid pB007"
     CDS             1..861
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1032..1620
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1908..1929
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        1944..1974
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1982..1998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2006..2022
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     mobile_element  2513..2547
                     /mobile_element_type="transposon"
                     /note="piggyBac transposon 5 terminal repeat"
     regulatory      2548..3195
                     /regulatory_class="promoter"
                     /gene="EF1a"
                     /note="Includes promoter region of Pseudococcomyxa sp. 
                     strain KJ nuclear gene EF1a for elongation factor 1 alpha 
                     subunit"
     gene            2548..3195
                     /gene="EF1a"
                     /label=EF1a
     regulatory      3190..3199
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3196..4701
                     /codon_start=1
                     /gene="UMPS"
                     /product="uridine monophosphate synthase"
                     /label=UMPS
                     /protein_id="BAY00745.1"
                     /translation="MATSTPSVATAAELKPKEGPITSQEFEELVLRLHEIEAVKFGNFK
                     LKSGLMSPIYIDLRVIVSYPDVLRRVAEVMWHQVSGAGFDVMCGVPYTALPIATCMSLL
                     HGTPMLMRRKEVKEYGTKKAIEGAFKKGQTCLIVEDLVTSGASVMETVEPLEVEGLKVA
                     DVVVLIDREQGGAARMASNGLRLHSAFTLSFIIKTLQAHGLVSAEVADSVAAFIAANQT
                     FSPSAAAAPTPASAPPAQPKRLPFGERAALCQNAAGRKLLELMARKRTNLAVAADVATV
                     EEMLRIADAAGPHIAVFKTHVDIFDEWDDGIAAQLRHLADKHEFLIFEDRKFADIGNTV
                     VSQYGGGIYKIADWSDITNAHLVPGPGIIDGLRKVGQEKGRGLLLLAEMSSKGALATGA
                     YTEKVAEAAAANQDFVMGFICQSPAKWATSVPPGLVHMTPGVQLASGSDALGQQYNTPA
                     SVIGQGGSDVIIVGRGIIKAADPAAAAAQYREAGWAAYEATLA"
     gene            3196..4701
                     /gene="UMPS"
                     /label=UMPS
     regulatory      4702..5398
                     /regulatory_class="terminator"
                     /gene="EF1a"
                     /note="Includes terminator region of Pseudococcomyxa sp. 
                     strain KJ nuclear gene EF1a for elongation factor alpha 
                     subunit"
     gene            4702..5398
                     /gene="EF1a"
                     /label=EF1a
     mobile_element  5399..5461
                     /mobile_element_type="transposon"
                     /note="piggyBac transposon 3 inverted terminal repeat"
     misc_feature    5462..6334
                     /label=derived from plasmid pB007
                     /note="derived from plasmid pB007"
     primer_bind     complement(5591..5607)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5749..6204
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6230..6334
                     /gene="bla"
                     /label=AmpR promoter