LOCUS       Exported                3585 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Fusion cloning vector pTRXFUS, complete sequence.
ACCESSION   U16857
VERSION     .
KEYWORDS    thioredoxin gene fusion vector.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   LaVallie ER, DiBlasio EA, Kovacic S, Grant KL, Schendel PF, McCoy 
            JM.
  TITLE     A thioredoxin gene fusion expression system that circumvents 
            inclusion body formation in the E. coli cytoplasm
  JOURNAL   Biotechnology (N.Y.) 11 (2), 187-193 (1993)
  PUBMED    7763371
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   LaVallie ER.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-1994) Edward R. LaVallie, Genetics Institute, 87 
            CambridgePark Drive, Cambridge, MA 02140, USA
REFERENCE   3  (bases 1 to 3585)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Cloning vector pTRXFUS"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:37161"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             201..1061
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="AAA67269.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      2159..2187
                     /regulatory_class="promoter"
                     /note="lambda pL promoter"
     CDS             2724..3128
                     /codon_start=1
                     /note="Derived from E. coli thioredoxin gene; normal 
                     translation termination codon following nucleotide 3050 has
                     been removed"
                     /protein_id="AAA67270.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLAGSGSGDDDDKVPGDPLESTCSNRTG"
     mat_peptide     2724..3050
                     /product="thioredoxin"
     CDS             2724..3050
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     misc_feature    3066..3080
                     /label=encodes enterokinase site
                     /note="encodes enterokinase site"
     CDS             3066..3080
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     misc_feature    3081..3112
                     /label=polylinker cloning site
                     /note="polylinker cloning site"
     regulatory      3113..3179
                     /regulatory_class="terminator"
                     /note="aspA transcription terminator"
     primer_bind     complement(3189..3205)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"